Transgenic knockout animal model null for pigment epithelium-derived factor (PEDF)
AssigneeNorthwestern University
InventorsNoel P. Bouck, Susan E. Crawford, Veronica Stellmach
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Abstract
The present invention relates to transgenic knockout animal models null for pigment epithelium-derived factor (PEDF). The present invention also provides methods for generating animal disease models and screening methods for identifying biologically active compounds.
Claims20
A transgenic knockout mouse whose genome comprises a homozygous or heterozygous disruption in its endogenous pigment epithelium-derived factor gene, wherein said homozygous disruption prevents the expression of a functional pigment eptithelium-derived factor protein, and wherein said heterozygous disruption reduces the expression of functional pigment eptithelium-derived factor protein in said knockout mouse.
A method for inducing cancer in a transgenic knockout mouse, comprising;
- a) providing a transgenic knockout mouse whose genome comprises a homozygous or heterozygous disruption in its endogenous pigment epithelium-derived factor gene, wherein said homozygous disruption prevents the expression of a functional pigment eptithelium-derived factor protein, and wherein said heterozygous disruption reduces the expression of functional pigment eptithelium-derived factor protein in said knockout mouse;
- and
- b) exposing said transgenic knockout mouse to conditions such that cancer formation is promoted in said transgenic knockout mouse.
Dependencies are inferred from the claim text.