Removal of extraneous substances from biological fluids containing nucleic acids and the recovery of nucleic acids
OwnerJohn Krupeyinventor
Life of this patent
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Abstract
A method for removing proteins and unwanted aggregated DNA from biological media containing nucleic acids by subjecting the starting material to a water insoluble complex consisting of ProCipitate™ and protein interspersed with ferric oxide particles to a magnetic force.
Claims15
A method for removing proteins and unwanted aggregated DNA from biological specimen containing nucleic acids, comprising:
- (A) contacting a specimen including nucleic acids with a water insoluble complex comprising (1) a water insoluble protein bridging network polyelectrolyte having an affinity to aggregate protein present in biological media while leaving DNA intact in a supernatant and (2) protein interspersed with (3) ferric oxide particles to form a mixture, and
- (B) applying a magnetic force to said mixture.
A method for removing proteins and aggregated DNA from biological specimens and removing the desired nucleic acids, comprising:
- contacting a specimen including nucleic acids to a water insoluble complex containing of (1) a water insoluble protein bridging network polyelectrolyte having an affinity to aggregate protein present in biological media while leaving DNA intact in a supernatant and (2) protein interspersed with (3) ferric oxide particles to form a mixture;
- or
- contacting a specimen including nucleic acids to a water insoluble complex comprising said protein network bridging polyelectrolyte aggregated DNA and protein interspersed with ferric oxide particles to form a mixture;
- or
- contacting a specimen including nucleic acids to a water insoluble complex comprising aggregated DNA and protein interspersed with ferric oxide to form a mixture;
- and
- applying a magnetic force to said mixture.
A method for removing proteins and aggregated DNA from biological specimens and recovering the desired nucleic acids, comprising:
- contacting a specimen including nucleic acids to a water insoluble complex containing of (1) a water insoluble protein bridging network polyelectrolyte having an affinity to aggregate protein present in biological media while leaving DNA intact in a supernatant and (2) protein interspersed with (3) a heavy metal oxide to form a mixture;
- or
- contacting a specimen including nucleic acids to a water insoluble complex comprising said protein network bridging polyelectrolyte aggregated DNA and protein interspersed with bismuth oxychloride;
- and
- allowing the complex to settle under unit gravity.
A method for removing proteins and unwanted aggregated DNA from a biological specimen containing nucleic acids, comprising:
- (A) contacting a specimen including nucleic acids with a water insoluble complex comprising aggregated DNA and protein interspersed with ferric oxide particles or bismuth oxychloride, to form a mixture, and
- (B) applying a magnetic force to said mixture.
Dependencies are inferred from the claim text.