Detection of rare Candida species
OwnerHermann Einsele, Jurgen Lofflerinventors
Life of this patent
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Abstract
The present invention relates to a nucleic acid molecule, to a kit which comprises the nucleic acid molecule, to methods for detecting fungi in clinical material and for determining the sequence of ribosomal fungal genes, and to kits for carrying out these methods.
Claims24
A nucleic acid molecule, which comprises a nucleotide sequence selected from the group consisting of:
- SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 7, SEQ ID No. 8, SEQ ID No. 9, SEQ ID No. 10, SEQ ID No. 11, SEQ ID No. 12, SEQ ID No. 13, SEQ ID No. 14, SEQ ID No. 15, SEQ ID No. 16, SEQ ID No. 17, and a sequence which binds to such a sequence to which one of the sequences SEQ IDs No. 1-4 or SEQ ID Nos. 7-17 binds.
A method for detecting a fungus in a clinical material, comprising:
- a) obtaining a fungal DNA from said clinical material,
- b) detecting the fungal DNA, wherein said detecting is performed using a nucleic acid molecule, which comprises a nucleotide sequence selected from the group consisting of:
- SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 7, SEQ ID No. 8, SEQ ID No. 9, SEQ ID No. 10, SEQ ID No. 11, SEQ ID No. 12, SEQ ID No. 13, SEQ ID No. 14, SEQ ID No. 15, SEQ ID No. 16, SEQ ID No. 17, and a sequence which binds to such a sequence to which one of the sequences SEQ IDs No. 1-4 or SEQ ID Nos. 7-17 binds.
A method for determining a sequence of a ribosomal fungal gene, comprising:
- a) obtaining a fungal DNA from a clinical material;
- b) detecting the fungal DNA, and
- c) sequencing the fungal DNA detected in step b),
- wherein said detecting is performed by using at least one nucleic acid molecule, which comprises a nucleotide sequence selected from the group consisting of:
- SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 7, SEQ ID No. 8, SEQ ID No. 9, SEQ ID No. 10, SEQ ID No. 11, SEQ ID No. 12, SEQ ID No. 13, SEQ ID No. 14, SEQ ID No. 15, SEQ ID No. 16, SEQ ID No. 17, and a sequence which binds to such a sequence to which one of the sequences SEQ IDs No. 1-4 or SEQ ID Nos. 7-17 binds.
A method for determining a sequence of a ribosomal fungal gene, comprising:
- a) obtaining a fungal DNA from a clinical material;
- b) detecting the fungal DNA, and
- c) sequencing the fungal DNA detected in step b),
- wherein said detecting is performed by amplification of a segment of the fungal DNA by a polymerase chain reaction {PCR) in which the PCR primer pair is selected from the group consisting of:
- SEQ ID Nos. 1 and 2;
- SEQ ID Nos. 3 and 4;
- SEQ ID NOS. 7 and 8;
- SEQ ID Nos. 9 and 10;
- and two sequences which bind to such sequences to which SEQ ID Nos. 1 and 2, or SEQ ID Nos. 3 and 4, or SEQ ID Nos. 7 and 8, or SEQ ID Nos. 9 and 10 bind.
A kit, comprising a nucleic acid molecule, which comprises a nucleotide sequence selected from the group consisting of:
- SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 7, SEQ ID No. 8, SEQ ID No. 9, SEQ ID No. 10, SEQ ID No. 11, SEQ ID No. 12, SEQ ID No. 13, SEQ ID No. 14, SEQ ID No. 15, SEQ ID No. 16, SEQ ID No. 17, and a sequence which binds to such a sequence to which one of the sequences SEQ IDs No. 1-4 or SEQ ID Nos. 7-17 binds.
Dependencies are inferred from the claim text.