IFN-alpha homologues
AssigneeMaxygen, Inc.
InventorsVolker Heinrichs, Teddy Chen, Phillip A. Patten
Life of this patent
Estimating the term…
Abstract
Alpha interferon homologues (both nucleic acids and polypeptides) are provided. Compositions including these interferon homologue polypeptides and nucleic acids, recombinant cells comprising said homologue polypeptides and nucleic acids, methods of making the new homologues, antibodies to the new homologues, and methods of using the homologues are provided. Integrated systems comprising the sequences of the nucleic acids or polypeptides are also provided.
Claims123
An isolated or recombinant nucleic acid, comprising:
- a polynucleotide sequence selected from the group consisting of:
- (a) SEQ ID NO:1 to SEQ ID NO:35, or a complementary polynucleotide sequence thereof;
- (b) a polynucleotide sequence encoding a polypeptide selected from SEQ ID NO:36 to SEQ ID NO:70, or a complementary polynucleotide sequence thereof;
- (c) a polynucleotide sequence which hybridizes under highly stringent conditions over substantially the entire length of polynucleotide sequence (a) or (b);
- and
- (d) a polynucleotide sequence comprising a fragment of (a), (b), or (c), which fragment encodes a polypeptide having antiproliferative activity in a human Daudi cell line-based assay.
An isolated or recombinant nucleic acid, comprising:
- a polynucleotide sequence selected from the group consisting of:
- (a) SEQ ID NO:72 to SEQ ID NO:78, or a complementary polynucleotide sequence thereof;
- (b) a polynucleotide sequence encoding a polypeptide selected from SEQ ID NO:79 to SEQ ID NO:85, or a complementary polynucleotide sequence thereof;
- (c) a polynucleotide sequence which hybridizes under highly stringent conditions over substantially the entire length of polynucleotide sequence (a) or (b);
- and
- (d) a polynucleotide sequence comprising a fragment of (a), (b) or (c), which fragment encodes a polypeptide having antiviral activity in a murine cell line/EMCV-based assay.
An isolated or recombinant nucleic acid, comprising:
- a polynucleotide sequence encoding a polypeptide, the polypeptide comprising the amino acid sequence:
- CDLPQTHSLG-X11-X12-RA-X15-X16-LL-X19-QM-X22-R-X24-S-X26-FSCLKDR-X34-DFG-X38-P-X40-EEFD-X45-X46-X47-FQ-X50-X51-QAI-X55-X56-X57-HE-X60-X61-QQTFN-X67-FSTK-X72-SS-X75-X76-W-X78-X79-X80-LL-X83-K-X85-X86-T-X88-L-X90-QQLN-X95-LEACV-X101-Q-X103-V-X105-X106-X107-X108-TPLMN-X114-D-X116-ILAV-X121-KY-X124-QRITLYL-X132-E-X134-KYSPC-X140-WEVVRAEIMRSFSFSTNLQKRLRRKE, or a conservatively substituted variation thereof, where X11 is N or D;
- X12 is R, S, or K;
- X15 is L or M;
- X16 is I, M, or V;
- X19 is A or G;
- X22 is G or R;
- X24 is I or T;
- X26 is P or H;
- X34 is H, Y or Q;
- X38 is F or L;
- X40 is Q or R;
- X45 is G or S;
- X46 is N or H;
- X47 is Q or R;
- X50 is K or R;
- X51 is A or T;
- X55 is S or F;
- X56 is V or A;
- X57 is L or F;
- X60 is M or I;
- X61 is I or M;
- X67 is L or F;
- X72 is D or N;
- X75 is A or V;
- X76 is A or T;
- X78 is E or D;
- X79 is Q or E;
- X80 is S, R, T, or N;
- X83 is E or D;
- X85 is F or L;
- X86 is S or Y;
- X88 is E or G;
- X90 is Y, H, N;
- X95 is D, E, or N;
- X101 is I, M, or V;
- X103 is E or G;
- X105 is G or W;
- X106 is V or M;
- X107 is E, G, or K;
- X108 is E or G;
- X114 is V, E, or G;
- X116 is S or P;
- X121 is K or R;
- X124 is F or L;
- X132 is T, I, or M;
- X134 is K or R;
- and X140is A or S.
An isolated or recombinant nucleic acid comprising a polynucleotide sequence encoding a polypeptide, the polypeptide comprising:
- an amino acid sequence comprising at least 20 contiguous amino acids of any one of SEQ ID NOS:36-70, and one or more of amino acids Ala19, (Tyr or Gln)34, Gly37, Phe38, Lys71, Ala76, Tyr90, Ile132, Arg134, Phe152, Lys160, and Glu166, wherein the numbering of the amino acids corresponds to that of SEQ ID NO:36.
An isolated or recombinant nucleic acid comprising a polynucleotide sequence encoding a polypeptide, the polypeptide comprising:
- an amino acid sequence comprising at least 155 contiguous amino acids of any one of SEQ ID NOS:36-70, said amino acid sequence comprising amino acids Lys160 and Glu166, wherein the numbering of the amino acids corresponds to that of SEQ ID NO:36.
An isolated or recombinant polypeptide, comprising:
- the amino acid sequence:
- CDLPQTHSLG-X11-X12-RA-X15-X16-LL-X19-QM-X22-R-X24-S-X26-FSCLKDR-X34-DFG-X38-P-X40-EEFD-X45-X46-X47-FQ-X50-X51-QAI-X55-X56-X57-HE-X60-X61-QQTFN-X67-FSTK-X72-SS -X75-X76-W-X78-X79-X80-LL-X83-K-X85-X86-T-X88-L-X90-QQLN-X95-LEACV-X101-Q-X103-V-X105-X106-X107-X108-TPLMN-X114-D-X116-ILAV-X121-KY-X124-QRITLYL-X132-E-X134-KYSPC-X140-WEVVRAEIMRSFSFSTNLQKRLRRKE, or a conservatively substituted variation thereof;
- wherein X11 is N or D;
- X12 is R, S, or K;
- X15 is L or M;
- X16 is I, M, or V;
- X19 is A or G;
- X22 is G or R;
- X24 is I or T;
- X26 is P or H;
- X34 is H, Y or Q;
- X38 is F or L;
- X40 is Q or R;
- X45 is G or S;
- X46 is N or H;
- X47 is Q or R;
- X50 is K or R;
- X51 is A or T;
- X55 is S or F;
- X56 is V or A;
- X57 is L or F;
- X60 is M or I;
- X61 is I or M;
- X67 is L or F;
- X72 is D or N;
- X75 is A or V;
- X76 is A or T;
- X78 is E or D;
- X79 is Q or E;
- X80 is S, R, T, or N;
- X83 is E or D;
- X85 is F or L;
- X86 is S or Y;
- X88 is E or G;
- X90 is Y, H, N;
- X95 is D, E, or N;
- X101 is I, M, or V;
- X103 is E or G;
- X105 is G or W;
- X106 is V or M;
- X107 is E, G, or K;
- X108 is E or G;
- X114 is V, E, or G;
- X116 is S or P;
- X121 is K or R;
- X124 is F or L;
- X132 is T, I, or M;
- X134 is K or R;
- and X140 is A or S.
A polypeptide comprising at least 100 contiguous amino acids of a protein encoded by a coding polynucleotide sequence, the polynucleotide sequence selected from the group consisting of:
- (a) SEQ ID NO:1 to SEQ ID NO:35 or SEQ ID NO:72 to SEQ ID NO:78;
- (b) a coding polynucleotide sequence that encodes a first polypeptide selected from SEQ ID NO:36 to SEQ ID NO:70 or SEQ ID NO:79 to SEQ ID NO:85;
- and
- (c) a complementary polynucleotide sequence which hybridizes under highly stringent conditions over substantially an entire length of a polynucleotide sequence of (a) or (b).
An isolated or recombinant polypeptide, comprising:
- an amino acid sequence comprising at least 50 contiguous amino acids of any one of SEQ ID NOS:36-70, the amino acid sequence comprising one or more of amino acids Ala19, (Tyr or Gln)34, Gly37, Phe38, Lys71, Ala76, Tyr90, Ile132, Arg134, Phe152, Lys160, and Glu166, wherein the numbering of the amino acids corresponds to that of SEQ ID NO:36.
An isolated or recombinant polypeptide comprising an amino acid sequence comprising at least 155 contiguous amino acids of any one of SEQ ID NOS:36-70, the isolated or recombinant polypeptide comprising amino acids Lys160 and Glu166, wherein the numbering of the amino acids corresponds to that of SEQ ID NO:36.
A polypeptide which is specifically bound by a polyclonal antisera raised against at least one antigen, said at least one antigen comprising at least one amino acid sequence of SEQ ID NO:36 to SEQ ID NO:70 or SEQ ID NO:79 to SEQ ID NO:85, or a fragment thereof, wherein the antisera is subtracted with an IFN-alpha polypeptide encoded by a nucleic acid corresponding to one or more of GenBank accession number:
- J00210 (alpha-D), J00207 (Alpha-A), X02958 (Alpha-6), X02956 (Alpha-5), V00533 (alpha-H), V00542 (alpha-14), V00545 (IFN-1B), X03125 (alpha-8), X02957 (alpha-16), V00540 (alpha-21), X02955 (alpha-4b), V00532 (alpha-C), X02960 (alpha-7), X02961 (alpha-10 pseudogene), R0067 (Gx-1), I01614, I01787, I07821, M12350 (alpha-F), M38289, V00549 (alpha-2a), and I08313 (alpha-Con1).
An antibody or antisera which specifically binds a polypeptide, the polypeptide comprising a sequence selected from the group consisting of:
- SEQ ID NO:36 to SEQ ID NO:70 or SEQ ID NO:79 to SEQ ID NO:85, wherein the antibody or antisera does not specifically bind to an IFN-alpha polypeptide encoded by a nucleic acid corresponding to one or more of GenBank accession number:
- J00210 (alpha-D), J00207 (Alpha-A), X02958 (Alpha-6), X02956 (Alpha-5), V00533 (alpha-H), V00542 (alpha-14), V00545 (IFN-1B), X03125 (alpha-8), X02957 (alpha-16), V00540 (alpha-21), X02955 (alpha-4b), V00532 (alpha-C), X02960 (alpha-7), X02961 (alpha-10 pseudogene), R0067 (Gx-1), I01614, I01787, I07821, M12350 (alpha-F), M38289, V00549 (alpha-2a), and I08313 (alpha-Con1).
A computer or computer readable medium comprising a database comprising a sequence record comprising one or more character strings corresponding to a nucleic acid or protein sequence selected from SEQ ID NO:1 to SEQ ID NO:85.
An integrated system comprising a computer or computer readable medium comprising a database comprising one or more sequence records, each of said sequence records comprising one or more character strings corresponding to a nucleic acid or protein sequence selected from SEQ ID NO:1 to SEQ ID NO:85, the integrated system further comprising a user input interface allowing a user to selectively view said one or more sequence records.
A method of using a computer system to present information pertaining to at least one of a plurality of sequence records stored in a database, said sequence records each comprising at least one character string corresponding to SEQ ID NO:1 to SEQ ID NO:85, the method comprising:
- determining a list of at least one character string corresponding to one or more of SEQ ID NO:1 to SEQ ID NO:85 or a subsequence thereof;
- determining which of said at least one character string of said list are selected by a user;
- and
- displaying each of the selected character strings, or aligning each of the selected character strings with an additional character string.
A nucleic acid which comprises a unique subsequence in a nucleic acid selected from SEQ ID NO:1 to SEQ ID NO:35 or SEQ ID NO:72 to SEQ ID NO:78, wherein the unique subsequence is unique as compared to a nucleic acid sequence of a known interferon-alpha nucleic acid sequence or a nucleic acid corresponding to any of GenBank accession number:
- J00210 (alpha-D), J00207 (Alpha-A), X02958 (Alpha-6), X02956 (Alpha-5), V00533 (alpha-H), V00542 (alpha-14), V00545 (IFN-1B), X03125 (alpha-8), X02957 (alpha-16), V00540 (alpha-21), X02955 (alpha-4b), V00532 (alpha-C), X02960 (alpha-7), X02961 (alpha-10 pseudogene), R0067 (Gx-1), I01614, I01787, I07821, M12350 (alpha-F), M38289, V00549 (alpha-2a), and I08313 (alpha-Con1).
A polypeptide which comprises a unique subsequence in a polypeptide selected from:
- SEQ ID NO:36 to SEQ ID NO:70 or SEQ ID NO:79 to SEQ ID NO:85, wherein the unique subsequence is unique as compared to a sequence of a known interferon-alpha polypeptide or a sequence of a polypeptide encoded by a nucleic acid corresponding to any of GenBank accession number:
- J00210 (alpha-D), J00207 (Alpha-A), X02958 (Alpha-6), X02956 (Alpha-5), V00533 (alpha-H), V00542 (alpha-14), V00545 (IFN-1B), X03125 (alpha-8), X02957 (alpha-16), V00540 (alpha-21), X02955 (alpha-4b), V00532 (alpha-C), X02960 (alpha-7), X02961 (alpha-10 pseudogene), R0067 (Gx-1), I01614, I01787, I07821, M12350 (alpha-F), M38289, V00549 (alpha-2a), and I08313 (alpha-Con1).
A target nucleic acid which hybridizes under stringent conditions to a unique coding oligonucleotide which encodes a unique subsequence in a polypeptide selected from:
- SEQ ID NO:36 to SEQ ID NO:70 or SEQ ID NO:79 to SEQ ID NO:85, wherein the unique subsequence is unique as compared to a sequence of a known interferon-alpha polypeptide or a sequence of a polypeptide encoded by a nucleic acid corresponding to any of GenBank accession number:
- J00210 (alpha-D), J00207 (Alpha-A), X02958 (Alpha-6), X02956 (Alpha-5), V00533 (alpha-H), V00542 (alpha-14), V00545 (IFN-1B), X03125 (alpha-8), X02957 (alpha-16), V00540 (alpha-21), X02955 (alpha-4b), V00532 (alpha-C), X02960 (alpha-7), X02961 (alpha-10 pseudogene), R0067 (Gx-1), I01614, I01787, I07821, M12350 (alpha-F), M38289, V00549 (alpha-2a), and I08313 (alpha-Con1).
Dependencies are inferred from the claim text.