Targeted delivery of genes encoding interferon
AssigneeThe Immune Response Corporation
InventorsHenry C. Chiou, Dennis J. Carlo
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Abstract
A molecular complex comprising a gene encoding interferon releasably linked to a conjugate of a nucleic acid binding agent and a ligand which binds to a component on the surface of a cell. In a preferred embodiment, the gene encodes human IFN-α, IFN-β, or IFN-γ. The complex can be used to obtain targeted expression of interferon in selected cells either in vivo or in vitro.
Claims29
A method of delivering a gene encoding interferon to a target cell in a mammal, resulting in expression of the gene as a protein and secretion of the protein by the cell, the method comprising administering to the mammal a molecular complex comprising a gene encoding interferon releasably linked to a conjugate of a nucleic acid binding agent and a ligand which binds to a component on the surface of the target cell, wherein the gene is operably linked to genetic regulatory elements necessary for expression, processing and secretion of the encoded protein by the target cell.
A molecular complex comprising a gene encoding interferon releasably linked to a conjugate of a nucleic acid binding agent and a ligand which binds to a component on the surface of a cell, the gene being linked to genetic regulatory elements necessary for expression, processing and secretion of the encoded product by the cell.
A method of recombinantly expressing interferon in culture comprising incubating a host cell with a molecular complex comprising a gene encoding interferon releasably linked to a conjugate of a nucleic acid binding agent and a ligand which binds to a component on the surface of the host cell, the gene being linked to genetic regulatory elements necessary for expression, processing and secretion of the encoded interferon protein by the host cell.
A method for treating hepatitis infection in a patient comprising administering to the patient a molecular complex comprising a gene encoding human interferon-α releasably linked to a conjugate of a polycation and a ligand which binds to the asialoglycoprotein receptor present on the surface of liver cells, the gene being in a form suitable for expression, processing and secretion of the encoded product by the liver cells.
Dependencies are inferred from the claim text.