Polyene macrolide derivatives, use for vectoring molecules
AssigneesUniversite Pierre et Marie Curie, Technical University of Gdansk
InventorsJacques Bolard, Christine Garcia, Olivier Seksek, Edward Borowski, Jolanta Grzybowska
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Abstract
A composition having a negatively charged molecule and a cationic polyene macrolide compound having two to four positive charges that reacts with the negatively charged molecule is described. This compound can be used to vector molecules and especially nucleic acids into cells.
Claims30
A composition comprising:
- a negatively charged molecule of interest;
- and
- a cationic polyene macrolide compound which is capable of interacting with said molecule, wherein said cationic polyene macrolide has from 2 to 4 positive charges.
The method according to claim 25, further comprising a step of forming complexes between said molecule and said compound.
A composition comprising:
- a negatively charged molecule of interest;
- and
- a cationic polyene macrolide compound capable of interacting with said molecule, wherein said polyene macrolide compound is a polyene macrolide antibiotic derivative that comprises one or more cationic functional groups bonded covalently to the carboxyl function of an aglycone group and/or to an amine function or functions of said polyene macrolide antibiotic.
A method for transferring negatively charged molecules into cells, said method comprising:
- incubating a composition with cells wherein said cells are selected from the group of fungal cells, parasitic protozoa cells, yeast cells and mammalian cells and wherein said composition comprises a negatively charged molecule of interest;
- and
- a cationic polyene macrolide compound which is capable of interacting with said molecule, wherein said cationic polyene macrolide has from 2 to 4 positive charges.
A composition comprising:
- a negatively charged molecule of interest;
- and
- a cationic polyene macrolide compound which is capable of interacting with said molecule, with the proviso that said cationic polyene macrolide compound is not amphotericin B methyl ester.
Dependencies are inferred from the claim text.