Nucleic acids encoding kringle 1-5 region fragments of plasminogen
OwnerM. Judah Folkman, Michael S. O'Reillyinventors
Life of this patent
Estimating the term…
Abstract
Fragments of an endothelial cell proliferation inhibitor and method of use therefor are provided. The endothelial proliferation inhibitor is a protein derived from plasminogen, or more specifically is an angiostatin fragment. The angiostatin fragments generally correspond to kringle structures occurring within the endothelial cell proliferation inhibitor. The endothelial cell inhibiting activity of these fragments provides a means for inhibiting angiogenesis of tumors and for treating angiogenic-mediated disease.
Claims15
A method of inhibiting endothelial cell proliferation comprising administering to an endothelial cell a proliferation inhibiting amount of a plasminogen fragment having an amino acid sequence substantially similar to the kringle 1-5 region of a plasminogen molecule.
A method of treating a mammal with an angiogenic-mediated disease comprising administering to the mammal a treatment effective amount of a plasminogen fragment having an amino acid sequence substantially similar to the kringle 1-5 region of a plasminogen molecule.
A therapeutic composition for inhibiting endothelial cell proliferation comprising a pharmaceutically acceptable excipient and a plasminogen fragment having an amino acid sequence substantially similar to the kringle 1-5 region of a plasminogen molecule.
A composition comprising an isolated nucleotide sequence that codes for a plasminogen fragment having an amino acid sequence substantially similar to the kringle 1-5 region of a plasminogen molecule.
A method of expressing an plasminogen fragment having an endothelial cell proliferation inhibiting activity and having an amino acid sequence substantially similar to the kringle 1-5 region of a plasminogen molecule, comprising transfecting in a mammalian cell a vector, wherein the vector contains a DNA sequence encoding said plasminogen fragment, and wherein the vector is capable of expressing angiostatin fragment when present in the cell.
Dependencies are inferred from the claim text.