Modulation of PTEN expression via oligomeric compounds

A method of selecting a double stranded oligomeric compound comprising the steps of;

• (a) contacting a PTEN RNA with one or more single stranded oligomeric compounds;
• (b) identifying the single stranded oligomeric compound which modulates the expression of the PTEN RNA;
• and
• (c) synthesizing a second single stranded oligomeric compound which is complementary to said single stranded oligomeric compound yielding a double stranded oligomeric compound as the selected double stranded oligomeric compound.

A method of identifying one or more target regions on a target RNA comprising the steps of;

• (a) contacting a PTEN RNA with one or more single stranded oligomeric compounds;
• (b) identifying the single stranded oligomeric compounds of (a) which modulate the expression of the target RNA;
• (c) synthesizing a second single stranded oligomeric compound which is complementary to the single stranded oligomeric compound of (b) and hybridizing the two strands thereby producing a double stranded oligomeric compound;
• (d) contacting said PTEN RNA with one or more of the double stranded oligomeric compounds of (c);
• and
• (e) identifying the double stranded oligomeric compounds of (d) which modulates the expression of the target RNA.

A method of identifying double stranded oligomeric compounds, said method comprising the steps of;

• (a) cloning one or more target regions from a PTEN RNA into a vector/plasmid construct;
• (b) transfecting said vector/plasmid into a cell;
• (c) contacting said cell with one or more candidate double stranded oligomeric compounds, said compounds having one strand hybridizable to said target region;
• and
• (d) identifying the double stranded oligomeric compounds which modulate the expression of the PTEN RNA.

A method for identifying an optimized expression modulator of PTEN RNA comprising the steps of:

• (a) contacting one or more candidate single stranded oligomeric compounds with one or more target regions of a PTEN RNA and identifying single stranded oligomeric compounds which modulate PTEN RNA expression;
• and
• (b) generating one or more candidate double stranded oligomeric compounds comprising single stranded oligomeric compounds identified in step (a), and contacting said candidate double stranded oligomeric compounds with said PTEN RNA;
• (c) identifying double stranded oligomeric compounds which modulate PTEN RNA expression as an optimized modulator of PTEN RNA expression.