Glucoamylase variants
AssigneeNovozymes A/S
InventorsBjarne Ronfeldt Nielsen, Allan Svendsen, Henrik Pedersen, Jesper Vind, Hanne Vang Hendriksen, Torben Peter Frandsen
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Abstract
The invention relates to a variant of a parent fungal glucoamylase, which exhibits altered properties, in particular improved thermal stability and/or increased specific activity.
Claims30
A variant of a parent glucoamylase, comprising an alteration at one or more of the following positions:
- 59, 66, 72, 119, 189, 223, 227, 313, 340, 342, 352, 379, 386, 393, 395, 402, 408, 416, 425, 427, 444, 486, 490, 494 wherein (a) the alteration is independently
- (i) an insertion of an amino acid downstream of the amino acid which occupies the position,
- (ii) a deletion of the amino acid which occupies the position, or
- (iii) a substitution of the amino acid which occupies the position with a different amino acid, (b) the variant has glucoamylase activity and (c) each position corresponds to a position of the amino acid sequence of the parent glucoamylase having the amino acid sequence of SEQ ID NO:
- 2.
A variant of a parent glucoamylase, comprising one or more of the following:
- V59A, L66V/R, T72I, S119P, I189T, Y223F, F227Y, N313G, S340G, K352R, S356G, T379A, S386K,N,R,P, A393R, S395R, Y402F, E408R, T416A,R,D,N,C,Q,G,H,I,L,K,M,F,P,S,E,W,Y,V preferably T416H, A425T, N427S/M, S444G S486G, T490A, T494P/A, wherein (a) the variant has glucoamylase activity and (b) each position corresponds to a position of the amino acid sequence of the parent glucoamylase having the amino acid sequence of SEQ ID NO:
- 2.
The cell according to claims 18, which is a strain from Aspergillus, in particular A. niger.
The cell according to claims 17-19, which is a strain from Talaromyces, in particular Talaromyces emersonii.
Dependencies are inferred from the claim text.