Alpha,2,8-sialyltransferase
AssigneeKyowa Hakko Kogyo Co., Ltd.
InventorsKatsutoshi Sasaki, Kazumi Miura, Nobuo Hanai, Tatsunari Nishi
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Abstract
The invention provides a novel α-2,8-sialyltransferase expressed by a gene cloned from animal cells, a cDNA coding for the α-2,8-sialyltransferase, a method of detecting, or suppressing the production of α-2,8-sialyltransferase by using the cDNA, a recombinant vector containing the DNA as an insert and cells harboring the recombinant vector as well as methods of preparing same. The α-2,8-sialyltransferase of the invention is useful, for example, in the production of carbohydrate chains having a useful physiological activity, for example the ganglioside GD3, and modifications thereof.
Claims23
α-2,8-Sialyltransferase, in isolated form, comprising the amino acid sequence encoded by (a) DNA comprising the base sequence defined in SEQ ID NO:1;
- (b) DNA containing a base sequence different from the base sequence defined in SEQ ID NO:1, the difference being due to the availability of a plurality of codons for one amino acid or to spontaneous mutation occurring in individual animals including human;
- and (c) DNA derived from the DNA defined in (a) or (b) by mutation including substitution, deletion or insertion mutation, that does not cause loss of α-2,8-sialyltransferase activity.
α-2,8-Sialyltransferase, in isolated form, comprising the amino acid sequence defined in SEQ ID NO:2.
A DNA comprising the base sequence defined in SEQ ID NO:1.
A recombinant vector comprising, as an insert, a DNA sequence comprising the base sequence defined in SEQ ID NO:1, or a base sequence homologous thereto.
The plasmid pUC119-WP1.
Escherichia coli JM105/pUC119-WP1R (FERM BP-4192).
An isolated nucleic acid sequence encoding α-2,8-sialyltransferase.
Dependencies are inferred from the claim text.