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US 2004/0002137 A1Published application
Human tissue urokinase type plasminogen activator production
AssigneeGlobal Biotech, Inc. a Taiwan corporation
InventorsPaul Porwen Hung, Bryan T. H. Wu
Life of this patent
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Abstract
This invention features a nucleic acid expression vector that includes a bicistronic coding unit that comprises a first segment that encodes a human tissue urokinase plasminogen activator protein and a second segment that encodes an amplifiable dominant selectable marker (e.g., dihydrofolate reductase); and a promoter (e.g., a cytomegalovirus promoter) operably linked to the bicistronic coding unit.
Claims21
A nucleic acid expression vector comprising:
- a bicistronic coding unit that comprises a first segment that encodes a human tissue urokinase plasminogen activator protein and a second segment that encodes an amplifiable dominant selectable marker;
- and
- a promoter operably linked to the bicistronic coding unit.
A nucleic acid expression vector comprising:
- a bicistronic coding unit that comprises a first segment that encodes a human tissue urokinase plasminogen activator protein comprising SEQ ID NO:1 and a second segment that encodes dihydrofolate reductase;
- and
- a cytomegalovirus promoter operably linked to the bicistronic coding unit.
A mammalian CHO host cell comprising a nucleic acid which comprises:
- a bicistronic coding unit that comprises a first segment that encodes a human tissue urokinase plasminogen activator protein and a second segment that encodes an amplifiable dominant selectable marker;
- and
- a promoter operably linked to the bicistronic coding unit.
Dependencies are inferred from the claim text.