Disruption of PQS synthesis using precursor analogs
OwnerEverett C. Pesci, James P. Colemaninventors
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Abstract
The present invention concerns methods of detecting bacterial infections as well as methods of treating such infections with compounds such as methyl anthranilate. The detection and treatment of Pseudomonas is particularly preferred.
Claims23
A method for detecting PQS production in bacterial cells, comprising the steps of:
- collecting a bacterial cell sample;
- culturing said cells in vitro;
- then
- collecting supernatant from said cultured cells;
- preparing an extract of said supernatant;
- and then
- exposing said extract to ultraviolet light, and then
- detecting blue fluorescence from said extract, wherein blue fluorescence of said extract indicates the production of PQS by said bacterial cells.
A method of determining the quantity of Psueodomonas present in a biological sample, comprising the steps of:
- providing a biological sample collected from a mammalian subject;
- and then
- detecting the quantity of PQS in said biological sample, with the quantity of PQS present in said biological sample directly correlated to the amount of Pseudomonas in said biological sample.
A method of treating a bacterial infection in a subject in need thereof, comprising administering to said subject a compound of Formula I, or a pharmaceutically acceptable salt thereof, in an amount effective to treat said bacterial infection;
- 3
- wherein:
- R1 is loweralkyl;
- R2 and R3 are each independently selected from the group consisting of H and loweralkyl;
- and
- R4, R5, R6, and R7 are each independently selected from the group consisting of halo, H, and loweralkyl.
Dependencies are inferred from the claim text.