High throughput methods and devices for assaying analytes in a fluid sample
OwnerJeffrey F. Regan, Randall Miller +1inventors
InventorsJeffrey F. Regan, Randall Miller, Carl Peck
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Abstract
Methods and devices are provided herein that are useful for qualitatively detecting the presence of, and/or quantitatively determining the amount of, one or more analytes in a relatively small microvolume fluid sample, including body-fluid samples and non-body fluid samples. High throughput techniques are also provided for evaluating multiple samples.
Claims55
A method for detecting one or more analytes, said method comprising:
- a) collecting a predetermined microvolume of a fluid sample comprising said one or more analytes on a microplatform housed with a microdevice;
- b) drying said microplatform;
- and
- c) detecting the presence or amount of one or more analytes on or from said microplatform, the presence or amount thereof indicating the presence or amount of said one or more analytes.
A method for detecting one or more analytes in a plurality of fluid samples, comprising:
- a) collecting for each fluid sample at least one predetermined microvolume of a fluid comprising said one or more analytes at a microplatform housed in a microdevice;
- b) placing each of said microplatforms in a solid surface device comprising a plurality of receiving elements, each adapted to receive one of said microplatforms;
- and
- c) detecting the presence or amount of said one or more analytes from each of said microplatforms, the presence or amount thereof being related to the presence or amount of said one or more analytes in each of said fluid samples.
A microdevice comprising:
- a) a microplatform housing;
- and
- b) a microplatform disposed within said microplatform housing, said housing being adapted such that at least a portion thereof mates with a receiving element in a solid surface device comprising a plurality of receiving elements.
A method for determining an analyte, said method comprising:
- a) providing in combination in an assay medium a sample suspected of containing said analyte with a reagent, which comprises an antibody for said analyte having substantially all available binding sites on said antibody bound to a labeled analyte, under conditions wherein said analyte in said sample can compete off said labeled analyte from said antibody and
- b) examining said antibody or said medium for the presence and or amount of said labeled analyte, the presence and/or amount thereof being related to the presence and/or amount of said analyte in said sample.
A method for determining an analyte in a sample suspected of containing said analyte, said method comprising:
- a) collecting said analyte on a microplatform,
- b) adding to said microplatform an enzyme reagent that is capable of binding to said analyte on said microplatform, and
- c) examining said microplatform for the amount of enzyme activity and relating said amount to the presence and/or amount of said analyte in said sample.
Dependencies are inferred from the claim text.