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US 2004/0002115 A1Published application
Method of identifying protein CAMs (constitutively active mutants)
OwnerPauline Fraissignes, Sabine Gratzer +1inventors
InventorsPauline Fraissignes, Sabine Gratzer, Ekkehard Leberer
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Abstract
The present invention relates to a method of identifying protein Constitutively Active Mutants (CAMs) and the use thereof.
Claims6
Method of identifying protein CAMs (constitutively active mutants) wherein
- a) a library of mutated sequences of a protein is generated,
- b) yeast cells are transformed with such library, and
- c) the respective protein CAM is identified.
Method of identifying protein CAMs (constitutively active mutants) wherein
- d) a library of mutated sequences of a protein is generated,
- e) yeast cells are co-transformed with the library and a linearized expression vector,
- f) the transformed yeast cells are selected for the repair of the plasmid, and
- g) protein CAMs are identified by determining the activity of the respective protein mutant.
1 more element
Method as claimed in one of the foregoing claims, wherein the protein is a mammalian protein.
Dependencies are inferred from the claim text.