Process for identifying modulators of G-protein-coupled receptors
OwnerEvi Kostenisinventor
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Abstract
The invention relates to a widely applicable process for identifying chemical compounds, which modulate G-protein-coupled receptors, by means of novel hybrid G-proteins with broad receptor specificity and very high expression and also to chemical compounds which can be identified by such a process.
Claims170
A process for identifying a chemical compound modifying the action of at least one G-protein-coupled receptor (GPCR)-dependent signal transduction pathway of an organism, wherein said process comprises the following steps:
- a) providing at least one cell which contains at least one GPCR-dependent signal transduction pathway and which produces one or more than one G-protein;
- b) providing at least one chemical compound to be studied;
- c) contacting the cell of a) with one or more of the chemical compounds of b);
- d) determining the quantitative or qualitative effect of the chemical compound or compounds of b) on the signal transduction pathway of the cell of a) by means of a signal transduction pathway-dependent measurable signal.
A polynucleotide sequence coding for a polypeptide having the property of a G-protein, wherein the polypeptide sequence is selected from:
- a) SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8;
- b) a sequence according to a) lacking one or more amino acids;
- c) a sequence according to a) having an additional one or more amino acids;
- and
- d) an allelic variant of a sequence according to a).
A polynucleotide comprising a polynucleotide sequence selected from:
- a) SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, the corresponding sequence complementary thereto;
- and
- b) a polynucleotide sequence hybridizing with a polynucleotide sequence according to a) under stringent conditions.
A protein having an amino acid sequence selected from SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, and SEQ ID NO:10.
Dependencies are inferred from the claim text.