Predicting the significance of single nucleotide polymorphisms (SNPs) using ensemble-based structural energetics
OwnerVince Hilser, Robert O. Foxinventors
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Abstract
The present invention relates to a protein database and methods of developing a protein database that contains identified single nucleotide polymorphisms that are relevant to protein function. Yet further, the present invention relates to utilizing the protein database to design protein pharmaceuticals that have optimized pharmaceutical properties.
Claims13
A protein database comprising nonhomologus proteins having identified single nucleotide polymorphisms that are relevant to the function of the protein.
A method of developing a protein database comprising the steps of:
- inputting high resolution structures of proteins;
- generating an ensemble of incrementally different conformational states by combinatorial unfolding of a set of predefined folding units in all possible combinations of each protein;
- determining the probability of each said conformational state;
- calculating a residue-specific connectivity of each said conformational state;
- and
- calculating a functional connectivity of each of said conformation state.
A system for developing a protein database having identified single nucleotide polymorphisms that are relevant to the function of the protein comprising
- a protein database having a data structure for protein data, said data structure including data fields for relevant single nucleotide polymorphisms;
- and
- a computer-based program for identifying protein data for said database, said program having
- an input module for receiving high resolution structure data for one or more proteins, and
- a processing module for determining the relevance of single nucleotide polymorphisms in the active site of one or more proteins and storing said data into said data fields of said protein database.
A database having a data structure which stores information defining relevant single nucleotide polymorphism groups, said database comprising:
- a field for storing a value of an amino acid name or amino acid abbreviation;
- and
- one or more classification fields for storing a value representing a numerical value for a relevant single nucleotide polymorphism.
A method of designing a protein pharmaceutical exhibiting optimized pharmaceutical properties comprising the steps of:
- i. obtaining a test data set of variants of the protein pharmaceutical, wherein the variants comprise single nucleotide polymorphisms;
- ii. preparing a library of ensemble derived properties for the test data set using a computer based method;
- iii. obtaining experimental data for a given property for each protein variant within the test data set;
- iv. deriving a parametric equation using the experimental data and the library of ensemble derived properties;
- and
- v. creating a protein pharmaceutical using the information obtained by the above steps to provide optimized pharmaceutical properties.
A method of identifying relevant single nucleotide polymorphisms comprising the steps of:
- inputting high resolution structures of proteins;
- generating an ensemble of incrementally different conformational states by combinatorial unfolding of a set of predefined folding units in all possible combinations of each protein;
- determining the probability of each said conformational state;
- calculating a residue-specific connectivity of each said conformational state;
- and
- calculating a functional connectivity of each of said conformation state.
Dependencies are inferred from the claim text.