Method and kit for discovering nucleic acids that encode desired functions

A method of discovering nucleic acids, in a biological or genomic sample, that encode a desired function, comprising:

• selecting a specific desired function;
• preparing nucleic acids from the sample, wherein the existence, presence, identity, properties, or function of at least one of the prepared nucleic acids is unknown to the experimenter performing the method;
• separating the prepared nucleic acids;
• treating the separated nucleic acids in vitro to obtain transcripts;
• treating the transcripts in vitro to obtain proteins;
• testing the proteins for the desired function;
• and
• identifying the nucleic acid that encodes the protein exhibiting the desired function.

A method of discovering nucleic acids, in a biological or genomic sample, that encode a desired RNA function, comprising:

• selecting a specific desired RNA function;
• preparing nucleic acids from the sample, wherein the existence, presence, identity, properties or function of at least one of the prepared nucleic acids is unknown to the experimenter performing the method;
• separating the prepared nucleic acids;
• treating the separated nucleic acids in vitro to obtain transcripts;
• testing the transcripts for the desired function;
• and
• identifying the nucleic acid that encodes the transcript exhibiting the desired function.

A kit for discovering nucleic acids, in a biological, genomic or cDNA sample, associated with desired functions, comprising:

• one or more containers;
• reagents for preparing nucleic acids from the sample, wherein at least one of the prepared nucleic acids is unknown to the experimenter using the kit;
• vectors in which the prepared nucleic acids can be inserted;
• reagents for inserting the prepared nucleic acids into the vectors to form recombinant vectors;
• reagents for separating the recombinant vectors;
• reagents for transcribing the separated recombinant vectors in vitro to obtain transcripts;
• optionally, reagents for translating the transcripts in vitro to obtain proteins;
• and
• reagents for testing the transcripts or proteins for a desired function.

A kit for discovering nucleic acids, in a biological or genomic sample, that encode desired functions, comprising:

• one or more containers;
• reagents for preparing nucleic acids from the sample, wherein the existence, presence, identity, properties or function of at least one of the prepared nucleic acids is unknown to the experimenter using the kit;
• vectors in which the prepared nucleic acids can be inserted;
• reagents for inserting the prepared nucleic acids into the vectors to form recombinant vectors;
• reagents for separating the recombinant vectors;
• reagents for transcribing the separated recombinant vectors in vitro to obtain transcripts;
• reagents for translating the transcripts in vitro to obtain proteins;
• and
• reagents for testing the proteins for a desired function.

A kit for discovering nucleic acids, in a biological or genomic sample, that encode desired RNA functions, comprising:

• one or more containers;
• reagents for preparing nucleic acids from the sample, wherein the existence, presence, identity, properties or function of at least one of the prepared nucleic acids is unknown to the experimenter using the kit;
• vectors in which the prepared nucleic acids can be inserted;
• reagents for inserting the prepared nucleic acids into the vectors to form recombinant vectors;
• reagents for separating the recombinant vectors;
• reagents for transcribing the separated recombinant vectors in vitro to obtain transcripts;
• and
• reagents for testing the transcripts for a desired RNA function.

A kit for discovering nucleic acids, in a biological or genomic sample, that encode desired functions, comprising:

• one or more containers;
• nucleic acids prepared from the sample, wherein the existence, presence, identity, properties or function of more than one of the prepared nucleic acids is unknown to the experimenter using the kit;
• recombinant vectors in which the unknown nucleic acids have been inserted;
• transcripts of the recombinant vectors;
• and
• one of either (i) reagents for translating the transcripts, (ii) proteins translated from the transcripts, (iii) reagents for testing the proteins for a desired function, (iv) or (v) a combination of any of (i)-(iv).

A kit for discovering nucleic acids, in a biological or genomic sample, that encode desired RNA functions, comprising:

• one or more containers;
• nucleic acids prepared from the biological or genomic sample, wherein the existence, presence, identity, properties or function of ore than one of the prepared nucleic acids is unknown to the experimenter using the kit;
• recombinant vectors in which the unknown nucleic acids have been inserted;
• transcripts of the recombinant vectors;
• and
• reagents for testing the transcripts for a desired RNA function.