Monitoring amplification with fret probes
AssigneeUniversity of Utah Research Foundation
InventorsCarl T. Wittwer, Randy P. Rasmussen, Marla Lay
Life of this patent
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Abstract
The present invention is directed to kits for monitoring a nucleic acid during amplification. More particularly, the present invention relates to kits comprising a first oligonucleotide labeled with a first fluorophore, a second oligonucleotide labeled with a second fluorophore, and components for amplifying the locus, wherein the first and second fluorophores comprise a fluorescence resonance energy transfer pair. Hybridization of at least the first oligonucleotide to the amplified locus places the first and second fluorophores in a resonance energy transfer relationship.
Claims18
A kit for amplification and analysis of a locus of a nucleic acid sample, comprising:
- a first oligonucleotide labeled with a first fluorophore;
- a second oligonucleotide labeled with a second fluorophore;
- and
- components for amplifying the locus;
- wherein the first and second fluorophores comprise a fluorescence resonance energy transfer pair, and the first and second oligonucleotides are configured such that hybridization of the first and second oligonucleotides to the locus places the first and second fluorophores in a resonance energy transfer relationship.
A kit for amplification and analysis of a locus of a nucleic acid sample, comprising:
- an amplification solution comprising
- a first oligonucleotide labeled with a first fluorophore;
- a second oligonucleotide labeled with a second fluorophore;
- and
- primers configured for amplifying the locus;
- wherein the first and second fluorophores comprise a fluorescence resonance energy transfer pair, and the first and second oligonucleotides are configured such that hybridization of the first and second oligonucleotides to the locus places the first and second fluorophores in a resonance energy transfer relationship.
A kit for amplification and analysis of a locus of a nucleic acid sample, comprising:
- an oligonucleotide labeled with a first fluorophore;
- a pair of primers configured for amplifying the locus, one of the primers labeled with a second fluorophore;
- and
- components for amplifying the locus;
- wherein the first and second fluorophores comprise a fluorescence resonance energy transfer pair, and the oligonucleotide is configured such that hybridization of the oligonucleotide to an amplified copy of the locus places the first and second fluorophores in a resonance energy transfer relationship.
Dependencies are inferred from the claim text.