Universal signal amplification tail
AssigneeBio-Rad Laboratories, Inc.
InventorsJason Liu, John Wesley Breneman III, Arlene Carillo, Craig Hixson
Life of this patent
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Abstract
The present invention resides in a detection agent that can be used to amplify a detection signal for a target molecule. The detection agent contains a linear first single-stranded nucleic acid attached to a capture moiety that specific binds the target molecule of interest. The first single-stranded nucleic acid includes a plurality of repeat sequences, each of which is non-homopolymeric and can specifically bind to a second single-stranded nucleic acid that has at least one detectable label. Related method for using the claimed detection agent to amplify a detection signal and a kit for this purpose are also disclosed.
Claims24
A detection agent, comprising a linear first single-stranded nucleic acid attached to a capture moiety that specifically binds to a target molecule, wherein said first single-stranded nucleic acid comprises a plurality of repeat sequences, each of which is a non-homopolymeric polynucleotide and specifically binds to a second single-stranded nucleic acid comprising a detectable label.
A method for amplifying detection signals for a target molecule, comprising the steps of:
- (a) contacting a detection agent with a sample, said detection agent comprising a linear first single-stranded nucleic acid attached to a capture moiety that specifically binds to a target molecule, wherein said first single-stranded nucleic acid comprises a plurality of repeat sequences, each of which is a non-homopolymeric polynucleotide and specifically binds to a second single-stranded nucleic acid comprising a detectable label;
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- (b) contacting said detection agent with said second single-stranded nucleic acid under suitable conditions such that said repeat sequences specifically bind to said second single-stranded nucleic acid.
A kit for amplifying detection signals for a target molecule, comprising a detection agent that comprises a linear first single-stranded nucleic acid, which is attached to a capture moiety that specifically binds to the target molecule, and a second single-stranded nucleic acid comprising a detectable label, wherein said first single-stranded nucleic acid comprises a plurality of repeat sequences, each of which is a non-homopolymeric polynucleotide and specifically binds to the second single-stranded nucleic acid.
Dependencies are inferred from the claim text.