Cancer-linked gene as target for chemotherapy
OwnerPaul E. Young, Reinhard Ebner +1inventors
InventorsPaul E. Young, Reinhard Ebner, Jeffrey W. Strovel
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Abstract
Cancer-linked gene sequences, and derived amino acid sequences, are disclosed along with processes for assaying potential antitumor agents based on their modulation of the expression of these cancer-linked genes. Also disclosed are antibodies that react with the disclosed polypeptides and methods of using the antibodies to treat cancerous conditions, such as by using the antibody to target cancerous cells in vivo for purposes of delivering therapeutic agents thereto. Also described are methods of diagnosis using the gene sequences.
Claims35
A process for identifying an agent that modulates the activity of a cancer-related gene comprising:
- (a) contacting a compound with a cell containing a gene that corresponds to a polynucleotide having a sequence selected from the group consisting of polynucleotide sequences of SEQ ID NO:
- 1, 3 and 5 and under conditions promoting the expression of said gene;
- and
- (b) detecting a difference in expression of said gene relative to when said compound is not present
- thereby identifying an agent that modulates the activity of a cancer-related gene.
A process for determining the cancerous status of a cell, comprising determining an increase in the level of expression in said cell of a gene that corresponds to a polynucleotide having a sequence selected from the group consisting of polynucleotide sequences of SEQ ID NO:
- 1, 3 and 5 wherein an elevated expression relative to a known non-cancerous cell indicates a cancerous state or potentially cancerous state.
An isolated polypeptide comprising an amino acid sequence homologous to an amino acid sequence selected from the group consisting of the polypeptide sequences of SEQ ID NO:
- 2, 4 and 6 wherein any difference between said amino acid sequence and the sequence of polypeptide sequences of SEQ ID NO:
- 2, 4 and 6 is due solely to conservative amino acid substitutions and wherein said isolated polypeptide comprises at least one immunogenic fragment.
An isolated polypeptide comprising an amino acid sequence selected from the group consisting of the polypeptide sequences of SEQ ID NO:
- 2, 4 and 6.
An antibody that reacts with a polypeptide comprising an amino acid sequence selected from the group consisting of polypeptide sequences of SEQ ID NO:
- 2, 4 and 6.
A process for treating cancer comprising contacting a cancerous cell in vivo with an agent having activity against an expression product encoded by a gene sequence selected from the group consisting of polynucleotide sequences of SEQ ID NO:
- 1, 3 and 5.
A method for producing test data with respect to the gene modulating activity of a compound comprising:
- (a) contacting a compound with a cell containing a gene that corresponds to a polynucleotide having a sequence selected from the group consisting of polynucleotide sequences of SEQ ID NO:
- 1, 3 and 5 and under conditions promoting the expression of said gene;
- and
- (b) detecting a difference in expression of said gene relative to when said compound is not present
- (c) and producing test data with respect to the gene modulating activity of said compound based on a decrease in the expression of the determined genes that correspond to SEQ ID NO:
- 1, 3 and 5 indicating gene modulating action.
Dependencies are inferred from the claim text.