Novel polypeptides having sequence similarity to GDNFR and nucleic acids encoding the same
AssigneeGenentech, Inc.
InventorsJ. Christopher Grimaldi, Somasekar Seshagiri, Jeremy Stinson, William I. Wood, Zemin Zhang
Life of this patent
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Abstract
The present invention is directed to novel polypeptides having sequence similarity to GDNFR and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention.
Claims44
An isolated nucleic acid molecule which comprises DNA having at least about 80% sequence identity to (a) a DNA molecule encoding a PRO34128 polypeptide comprising the sequence of amino acid residues from about 1 to about 394 of FIG. 2 (SEQ ID NO:2), or (b) the complement of the DNA molecule of (a).
An isolated nucleic acid molecule comprising DNA which comprises at least about 80% sequence identity to (a) a DNA molecule encoding the same mature polypeptide encoded by the human protein cDNA deposited with the ATCC on Jan. 30, 2001 under ATCC Deposit No. PTA-2985 (DNA 194917-3044), or (b) the complement of the DNA molecule of (a).
An isolated nucleic acid molecule comprising DNA which comprises at least about 80% sequence identity to (a) the full-length polypeptide coding sequence of the human protein cDNA deposited with the ATCC on Jan. 30, 2001 under ATCC Deposit No. PTA-2985 (DNA 194917-3044), or (b) the complement of the coding sequence of (a).
An isolated nucleic acid molecule encoding a PRO34128 polypeptide comprising DNA that hybridizes to the complement of the nucleic acid sequence that encodes amino acids 1 to about 394 of FIG. 2 (SEQ ID NO:2).
An isolated nucleic acid molecule comprising at least about 707 nucleotides and which is produced by hybridizing a test DNA molecule under stringent hybridization conditions with (a) a DNA molecule which encodes a PRO34128 polypeptide comprising a sequence of amino acid residues from 1 to about 394 of FIG. 2 (SEQ ID NO:2), or (b) the complement of the DNA molecule of (a), and isolating the test DNA molecule.
A nucleic acid molecule deposited with the ATCC under accession number PTA-2985 (DNA 194917-3044).
An isolated PRO34128 polypeptide comprising an amino acid sequence comprising at least about 80% sequence identity to the sequence of amino acid residues from about 1 to about 394 of FIG. 2 (SEQ ID NO:2).
An isolated PRO34128 polypeptide having at least about 80% sequence identity to the polypeptide encoded by the cDNA insert of the vector deposited with the ATCC on Jan. 30, 2001 as ATCC Deposit No. PTA-2985 (DNA 194917-3044).
An isolated PRO34128 polypeptide comprising the sequence of amino acid residues from 1 to about 394 of FIG. 2 (SEQ ID NO:2), or a fragment thereof sufficient to provide a binding site for an anti-PRO34128 antibody.
An isolated polypeptide produced by (i) hybridizing a test DNA molecule under stringent conditions with (a) a DNA molecule encoding a PRO34128 polypeptide comprising the sequence of amino acid residues from 1 to about 394 of FIG. 2 (SEQ ID NO:2), or (b) the complement of the DNA molecule of (a), (ii) culturing a host cell comprising said test DNA molecule under conditions suitable for the expression of said polypeptide, and (iii) recovering said polypeptide from the cell culture.
A chimeric molecule comprising a PRO34128 polypeptide fused to a heterologous amino acid sequence.
An antibody which specifically binds to a PRO34128 polypeptide.
An agonist to a PRO34128 polypeptide.
An antagonist to a PRO34128 polypeptide.
A composition of matter comprising (a) a PRO34128 polypeptide, (b) an agonist to a PRO34128 polypeptide, (c) an antagonist to a PRO34128 polypeptide, or (d) an anti-PRO34128 antibody in admixture with a pharmaceutically acceptable carrier.
An isolated nucleic acid molecule which comprises a nucleotide sequence having at least about 80% sequence identity to (a) a DNA molecule encoding amino acids 1 to X of FIG. 2 (SEQ ID NO:2), where X is any amino acid from 347-377 of FIG. 2 (SEQ ID NO:2), or (b) the complement of the DNA molecule of (a).
An isolated nucleic acid molecule which comprises (a) a nucleotide sequence encoding a polypeptide scoring at least about 80% positives when compared with an amino acid sequence of residues from about 1 to X of FIG. 2 (SEQ ID NO:2), where X is any amino acid from 347-377 of FIG. 2 (SEQ ID NO:2), or (b) the complement of the nucleotide sequence of (a).
An isolated soluble PRO34128 polypeptide comprising an amino acid sequence having at least about 80% sequence identity to amino acids 1 to X of FIG. 2 (SEQ ID NO:2), where X is any amino acid from 347-377 of FIG. 2 (SEQ ID NO:2).
An isolated soluble PRO34128 polypeptide comprising an amino acid sequence which scores at least about 80% positives when compared with the amino acid sequence of amino acids 1 to X of FIG. 2 (SEQ ID NO:2), where X is any amino acid from 347-377 of FIG. 2 (SEQ ID NO:2).
Dependencies are inferred from the claim text.