US 2004/0002081 A1 · Method and device for isolating and purifying a polynucleotide of interest on a manufacturing scale

Abstract

The invention relates to a process for isolating and purifying a polynucleotide on a manufacturing scale which uses a chromatographic separation process comprising a combination of two different chromatographic steps selected from hydrophobic interaction chromatography, polar interaction chromatography and anion exchange chromatography. In at least one of the two steps the chromatographic support is a porous monolithic bed. The invention also relates to a chromatographic device and its use for isolating and purifying a polynucleotide of interest, in particular plasmid DNA, on manufacturing scale.

Claims23

Dependent claims

A process for isolating and purifying a polynucleotide of interest on a manufacturing scale, wherein a preparation containing said polynucleotide of interest is subjected to a chromatographic separation process, characterized in that

a) the chromatographic separation process comprises a combination of two steps that are based on different chromatographic principles, and in that
b) either of the two steps is selected from
i) hydrophobic interaction chromatography (HIC),
ii) polar interaction chromatography (PIC) and
iii) anion exchange chromatography (A-IEX);
and
in that
c) in at least one of the two steps the chromatographic support is a porous monolithic bed.

5 more elements

Dependencies are inferred from the claim text.

Facts

Application

10/321,792Filed 18 Dec 2002

Priority

EP EP 01 130 067.018 Dec 2001

Published

1 Jan 2004

Term ends

Not estimatedThe source records don’t support an estimate.

Classification10 codes

C12N 15/101
By means of a solid support carrier, e.g. particles, polymers: by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
B01D 15/322
Bonded phase chromatography: normal bonded phase
B01D 15/327
Reversed phase: with hydrophobic interaction
B01D 15/34
Characterised by the separation mechanism: size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation

Method and device for isolating and purifying a polynucleotide of interest on a manufacturing scale

Life of this patent

Method and device for isolating and purifying a polynucleotide of interest on a manufacturing scale

Life of this patent

Abstract

Claims23