Heat-resistant cysteine synthase
OwnerKazuhiko Ishikawa, Koshiki Minoinventors
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Abstract
The inventor isolated heat-resistant cysteine synthase having substantially no decline in activity after heat treatment at 80° C. for 6 hours from the hyperthermophilic archaea Aeropyrum pernix, and determined the amino acid sequence and base sequence. The DNA encoding this enzyme is DNA consisting of the base sequence set forth in SEQ ID NO: 1. The enzyme has the amino acid sequence set forth in SEQ ID NO: 2. The enzyme can be used to carry out an efficient enzyme reaction on highly concentrated substrates prepared at elevated temperatures.
Claims9
Heat-resistant cysteine synthase of which enzymatic activity is substantially unimpaired by heat treatment at 80° C. for 6 hours.
Heat-resistant cysteine synthase with at least 80% residual activity after heat treatment at 100° C. for 6 hours.
A polypeptide of (1) or (2) below:
- (1) a polypeptide comprising the amino acid sequence set forth in SEQ ID No:
- 2.
- (2) a polypeptide which comprises the amino acid sequence in SEQ ID NO:
- 2 but containing one or more deletions, substitutions or additions of amino acid residues and has heat resistant cysteine synthase activity.
Dependencies are inferred from the claim text.