Toxin detection and compound screening using biological membrane microarrays
OwnerYe Fang, Anthony G. Frutos +1inventors
InventorsYe Fang, Anthony G. Frutos, Joydeep Lahiri
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Abstract
A microarray containing components of cell membranes and a high-throughput method for using the microarray to detect toxins and screen for other biological or chemical compounds that may block the binding of toxin molecules to targets is provided. The microarray according to the present invention provides an attractive platform for efficient study of fundamental aspects of molecular recognition at the cell surface. Specific binding pattern of a given toxin to a set of different biological membrane probes in the microarray can be employed as a “signature” to identify and detect the presence of a toxin in a sample.
Claims50
A method for detecting and identifying a toxin in a sample, the method comprises:
- providing an array having a plurality of biological membranes associated with a surface of a substrate;
- contacting the array with a solution having a target compound;
- monitoring for binding activity of at least one biological membrane with said target compound.
The method according to claim 8, wherein the toxin-binding receptor is a sodium channel, a potassium channel, a calcium channel, and any combination of ion channels, an acetylcholine receptor, a ryanodine receptor, a glutamate receptor, a ceramide, a ganglioside, a cerebroside, sulfatides or cholesterol.
An array for identifying and detecting a toxin, the array comprising a plurality of biological membrane probes associated with a surface of a substrate;
- said biological membrane containing a toxin-binding moiety.
A method for detecting a binding event between a probe and target compound, said method comprising:
- providing an array having a plurality of biological membrane microspots associated with a surface of a substrate;
- contacting a solution comprising a target compound with said array of probe biological membrane microspots;
- and detecting a binding event between at least one or more of the probe microspots with one or more of the constituents of the target compound.
A method for identifying and detecting a toxin in a sample, said method comprising:
- providing an array having a plurality of biological membrane microspots associated with a surface of a substrate;
- contacting a sample solution comprising an unknown toxin with said array of biological membrane microspots;
- and detecting the binding profile of the unknown toxin to at least one or more of the microspots.
Dependencies are inferred from the claim text.