Retrovirus-based genomic screening

A vector comprising a genomic DNA sequence flanked by retroviral 5′ and 3′ long terminal repeats.

A vector library comprising a plurality of genomic DNA sequences flanked by retroviral 5′ and 3′ long terminal repeats, wherein said plurality of genomic DNA sequences are from about 10 to 200 kilobases in length.

A process for producing a retroviral particle comprising

• a. providing a vector and a packaging cell, wherein said vector comprises retroviral 5′ and 3′ long terminal repeats flanking a genomic DNA sequence;
• b. introducing said vector into said cell under conditions such that a RNA molecule corresponding to said retroviral 5′ and 3′ long terminal repeats flanking a genomic DNA sequence is transcribed and packaged into a retroviral particle.

A retroviral particle comprising a genome, said genome comprising 5′ and 3′ viral long terminal repeats flanking an exogenous RNA sequence, wherein said exogenous RNA sequence comprises an intron.

A host cell comprising a provirus comprising 5′ and 3′ viral long terminal repeats flanking an exogenous DNA sequence, wherein said exogenous DNA sequence comprises an intron.

A method of removing introns from genomic DNA, comprising

• a. providing a vector and a packaging cell, wherein said vector comprises retroviral 5′ and 3′ long terminal repeats flanking a genomic DNA sequence, said genomic sequences comprising one or more introns;
• b. introducing said vector into said cell under conditions such that a RNA molecule corresponding to said retroviral 5′ and 3′ long terminal repeats flanking a genomic DNA sequence is transcribed and packaged into a retroviral particle;
• wherein said RNA molecule lacks introns.