Signal for packaging of influenza virus vectors

An influenza viral vector, comprising influenza virus incorporation sequences, which vector comprises:

• a) sequences corresponding to the 3′ noncoding region of influenza virus HA vRNA, 3′ NA incorporation sequences, a heterologous nucleic acid segment, and the 5′ noncoding region of NA vRNA;
• b) sequences corresponding to the 3′ noncoding region of influenza virus HA vRNA, a heterologous nucleic acid segment, 5′ HA incorporation sequences, and the 5′ noncoding region of HA vRNA;
• c) sequences corresponding to the 3′ noncoding region of influenza virus M vRNA, 3′ M incorporation sequences, a heterologous nucleic acid segment, 5′ M incorporation sequences, and the 5′ noncoding region of M vRNA;
• d) sequences corresponding to the 3′ noncoding region of influenza virus NS vRNA, 3′ NS incorporation sequences, a heterologous nucleic acid segment, and the 5′ noncoding region of NS vRNA;
• e) sequences corresponding to the 3′ noncoding region of influenza virus PB2 vRNA, a heterologous nucleic acid segment, 5′ PB2 incorporation sequences, and the 5′ noncoding region of PB2 vRNA;
• f) sequences corresponding to the 3′ noncoding region of influenza virus PB1 vRNA, a heterologous nucleic acid segment, 5′ PB1 incorporation sequences, and the 5′ noncoding region of PB1 vRNA;
• or
• g) sequences corresponding to the 3′ noncoding region of influenza virus PA vRNA, a heterologous nucleic acid segment, 5′ PA incorporation sequences, and the 5′ noncoding region of PA vRNA;
• wherein vRNA corresponding to the vector, when present in a cell expressing influenza virus proteins and comprising vRNAs other than the vRNA corresponding to the vector, is packaged into virions.