PNA oligomers, oligomer sets, methods and kits pertaining to the detection of Bacillus anthracis
AssigneeBoston Probes, Inc.
InventorsHenrik Stender, Jens J. Hyldig-Nielsen
Life of this patent
Estimating the term…
Abstract
This invention is related to novel PNA probes, probe sets, methods and kits pertaining to the determination of Bacillus anthracis.
Claims59
A PNA oligomer comprising a probing nucleobase sequence, wherein at least a portion of the probing nucleobase sequence is at least ninety percent homologous to the nucleobase sequences, or their complements, selected from the group consisting of:
- CCA-SSG-GTA-TCD-DTC (Seq. ID No. 1) and TTC-AAA-GGC-TCC-CGC (Seq. ID No. 2).
A PNA oligomer that:
- a) comprises one or more non-natural nucleobases;
- b) does not substantially intra or inter molecularly self-hybridize;
- and
- c) sequence-specifically hybridizes to a region of the nucleic acid of Bacillus anthracis.
An oligomer set comprising two or more oligomers, at least one of which is a PNA oligomer comprising a probing nucleobase sequence that is at least ninety percent homologous to the nucleobase sequences, or their complements, selected from the group consisting of:
- CCA-SSG-GTA-TCD-DTC (Seq. ID No. 1) and TTC-AAA-GGC-TCC-CGC (Seq. ID No. 2).
28 The oligomer set of claim 25, wherein at least one oligomer of the set is labeled with at least one detectable moiety.
The oligomer set of claim 28, wherein the detectable moiety or moieties are each independently selected from the group consisting of:
- a dextran conjugate, a branched nucleic acid detection system, a chromophore, a fluorophore, a spin label, a radioisotope, an enzyme, a hapten, an acridinium ester and a chemiluminescent compound.
A method for detecting, identifying and/or quantitating Bacillus anthracis, or the rRNA or rDNA associated therewith, in a sample;
- said method comprising:
- a) contacting the sample, under suitable hybridization conditions, with at least one PNA oligomer that:
- i) comprises one or more non-natural nucleobases;
- ii) does not substantially intra or inter molecularly self-hybridize;
- and
- iii) is capable of sequence-specifically hybridizing to a target sequence within the rRNA of Bacillus anthracis;
- and
- b) detecting, identify and/or quantitating hybridization of the probing nucleobase sequence of the PNA oligomer to the target sequence and correlating the result with the presence, absence and/or quantity of Bacillus anthracis, or the rRNA or rDNA thereof, in the sample.
A method for detecting, identifying and/or quantitating Bacillus anthracis, or the rRNA or rDNA associated therewith, in a sample;
- said method comprising:
- a) contacting the sample, under suitable hybridization conditions, with at least one PNA oligomer comprising a probing nucleobase sequence that is at least ninety percent homologous to the nucleobase sequences, or their complements, selected from the group consisting of:
- CCA-SSG-GTA-TCD-DTC (Seq. ID No. 1) and TTC-AAA-GGC-TCC-CGC (Seq. ID No. 2);
- and
- b) detecting, identify and/or quantitating hybridization of the probing nucleobase sequence of a PNA oligomer to a target sequence within the nucleic acid of Bacillus anthracis and correlating the result with the presence, absence and/or quantity of Bacillus anthracis in the sample.
A kit suitable for determining the presence, absence and/or quantity of the nucleic acid of Bacillus anthracis in a sample, said kit comprising:
- a) one or more PNA oligomers comprising a probing nucleobase sequence that is at least ninety percent homologous to the nucleobase sequences, or their complements, selected from the group consisting of:
- CCA-SSG-GTA-TCD-DTC (Seq. ID No. 1), TTC-AAA-GGC-TCC-CGC (Seq. ID No. 2) and TTC-AAT-GGC-TCC-CGC (Seq. ID No. 3);
- and
- b) other reagents, instructions or compositions useful in performing the assay.
Dependencies are inferred from the claim text.