Bacterial test method by glycated label binding
OwnerMichael J. Pugia, Manju Basu +2inventors
InventorsMichael J. Pugia, Manju Basu, Robert Hatch, James Profitt
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Abstract
A method for measuring the bacteria content of fluids such as urine and blood, in which a glycoprotein or glycopeptide is attached to the bacteria and a label attached to or inherent to the glycoprotein or glycopeptide provides a means for determining the amount of bacteria present. A preferred glycoprotein is alkaline phosphatase, which is an enzyme capable of attaching to all bacteria present in the fluid sample and inherently includes a label moiety in that color can be developed by addition of known reagents.
Claims35
A method for measuring the bacteria content of fluids comprising:
- a binding an effective amount of a glycoprotein or glycopeptide with bacteria contained in a sample of fluid, said glycoprotein or glycopeptide having a label to indicate its presence;
- b. separating excess unbound glycoprotein or glycopeptide from said fluid sample after reacting said glycoprotein or glycopeptide with bacteria in said sample in step (a);
- c. measuring the amount of said label remaining after separating said excess unbound glycoprotein or glycopeptide of (b);
- and
- d. determining the bacteria content of said sample as related to the amount of said label measured in step (c).
A device for measuring the bacterial content of fluids comprising:
- a. a glycoprotein or glycopeptide labeled to provide a means for detecting said glycoprotein or glycopeptide;
- b. a structural support for said labeled glycoprotein or glycopeptide, whereby said labeled glycoprotein or glycopeptide can be brought into contact with a sample of said fluid.
Dependencies are inferred from the claim text.