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OwnerJoseph Remick, Anthony J.D. Feurtado +3inventors
InventorsJoseph Remick, Anthony J.D. Feurtado, Maryam Mobed-Miremadi, Tim J. Lazaruk, Jay K. Bass
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Methods and apparatus are disclosed for synthesizing a plurality of compounds on the surface of supports. Biopolymer features are attached to the surfaces of the supports. The synthesis generally comprises a plurality of steps. The support is placed into a flow chamber, and a reagent is introduced into the flow chamber. The reagent is reactive with features on the surface of the support. During removal of the reagent from the flow chamber, the pressure in the chamber is maintained substantially atmospheric. In another embodiment the reagent is removed from the flow chamber under vacuum. In another embodiment the reagent is removed from the flow chamber by simultaneously venting and applying a vacuum to the flow chamber.
A method for synthesizing a plurality of biopolymers on the surface of a support, said method comprising:
A method for synthesizing an array of biopolymers on the surface of a support wherein said synthesis comprises a plurality of monomer additions, said method comprising after each of said monomer additions:
17 A method according to claim 15 wherein said method further comprises holding said liquid reagent in said flow chamber for a predetermined period of time.
A flow cell assembly for conducting at least one reaction in the synthesis of an array of biopolymers on the surface of a support, said flow cell comprising:
Dependencies are inferred from the claim text.