Stabilized formulations of adenovirus

A method for stabilizing a composition comprising an Adenovirus, comprising adding to the composition a stabilizing-effective amount of a non-ionic detergent of Formula I

• R—O(CH2CH2O)X—H I
• wherein X is 4-30, and
• R is a linear or branched alkyl of 10-70 carbon atoms,
• or Formula II
• 24
• wherein R is a —CO2R′ having 10 to 70 carbons, and W+X+Y+Z=20, wherein R′ is as defined above for R,
• or Formula III
• 25
• wherein R is as above, and X is 5-100, Y is 25-75 and Z is 50-100,
• or Formula IV
• 26
• wherein X is 5-15, ring A is phenylene or cyclohexylene, and R′ is R as above,
• or a combination thereof.

A method for stabilizing a composition comprising Adenovirus, comprising adding to the composition a stabilizing-effective amount of a non-ionic detergent of Formula I

• R—O(CH2CH2O)X—H I
• wherein X is 4-30, and R is (CH3)(CH2)Y—, wherein Y is 10-15,
• or Formula II
• 27
• wherein R is CaH(2a+1)CO2—, a is 10 to 70,
• or Formula III
• 28
• wherein R is CH3, X is 55, Y is 29 and Z is 55, or R is CH3, X is 98, Y is 67 and Z is 98,
• or Formula IV,
• 29
• wherein R′ is (CH3)3C.CH2C(CH3)2—,A is phenylene, and X is 9-10 or R′ (CH3)3C.C2C(CH3)2—,
• A is cyclohexylene, and X is 9-10,
• or a combination thereof.

A method to stabilize a composition comprising Adenovirus, comprising adding to the composition a stabilizing-effective amount of a non-ionic detergent of Formula I

• R—O(CH2CH2O)X—H I
• wherein R is (CH3)(CH2)Y—, and
• wherein X is 23 and Y is 12, or X is 20 and Y is 12,
• or Formula II
• 30
• wherein R is R═C11H23CO2—,
• or a combination thereof.

A method for stabilizing a composition comprising an airborne virus, comprising adding to the composition a stabilizing-effective amount of a non-Ionic detergent of Formula I

• R—O(CH2CH2O)X—H I
• wherein X is 4-30, and R is a linear or branched alkyl of 10-70 carbon atoms,
• or Formula II
• 31
• wherein R is a —CO2R′ having 10 to 70 carbons, and W+X+Y+Z=20, wherein R′ is as defined above for R,
• or Formula III
• 32
• wherein R is as above, and X is 5-100, Y is 25-75 and Z is 50-100,
• or Formula IV.
• 33
• wherein X is 5-15, ring A is phenylene or cyclohexylene, and R′ is R as above,
• or a combination thereof, in a concentration ranging from about 0.005% to about 0.1% (vol/vol).

A method for stabilizing a composition comprising an airborne virus, comprising adding to the composition a stabilizing-effective amount of a non-ionic detergent of Formula I.

• R—O(CH2CH2O)X—H I
• wherein X is 4-30, and R is (CH3)(CH2)Y—, wherein Y is 10-15,
• or Formula III
• 34
• wherein R is CaH(2a+1)CO2—, a is 10 to 70,
• or Formula III
• 35
• wherein R is CH3, X is 55 Y is 29 and Z is 55, or R is CH3, X is 98, Y is 67 and Z is 98,
• or Formula IV,
• 36
• wherein R′ is (CH3)3C.CH2C(CH3)2—, A is phenylene, and X is 9-10, or R′ (CH3)3C.CH2C(CH3)2—,
• A is cyclohexylene, and X is 9-10,
• or a combination thereof.

A method to stabilize a composition comprising airborne virus, comprising adding to the composition a stabilizing-effective amount of a non-ionic detergent of Formula I

• R—O(CH2CH2O)X—H I
• wherein R is (CH3(CH2)Y—, and wherein X is 23 and Y is 12, or X is 20 and Y is 12,
• or Formula II
• 37
• wherein R is R═C11H23CO2—,
• or a combination thereof.

A method for stabilizing a composition comprising an airborne virus to reduce loss of virus amount or activity comprising adding to the composition a stabilizing-effective amount of a non-ionic detergent of Formula I

• R—O(CH2CH2O)X—H I
• wherein
• X is 4-30, and R is a linear or branched alkyl of 10-70 carbon atoms,
• or Formula II
• 38
• wherein R is —CO2R′ having 10 to 70 carbons, and W+X+Y+Z=20, wherein R′ is as above for R,
• or Formula III
• 39
• wherein R is as above, and X is 5-100, Y is 25-75 and Z is 50-100,
• or Formula IV
• 40
• wherein X is 5-15 ring A is phenylene or cyclohexylene, and R′ is R as above, or a combination thereof, wherein the loss of virus amount or activity is less than about 30% over a given period of time at about 2-8° C., room temperature, 37° C., −20° C. or −70° C. compared to the loss when said non-ionic detergent is not present.

A pharmaceutical composition comprising an airborne virus a stabilizing-effective amount of a non-ionic detergent of Formula I,

• R—O(CH2 CH2O)X—H I
• wherein
• X is 4-30 and R is a linear or branched alkyl of 10-70 carbon atoms,
• or Formula II
• 41
• wherein R IS —CO2R′ having 10 to 70 carbons, and W+X+Y+Z=20, wherein R′ is as above for R,
• or Formula III
• 42
• wherein R is as above, and X is 5-100, Y is 25-75 and Z is 50-100,
• or Formula IV
• 43
• wherein X is 5-15 ring A is phenylene or cyclohexylene, and R′ is R as above,
• or a combination thereof and at least one pharmaceutically acceptable carrier, salt or excipient.

A pharmaceutical composition, comprising

• a) an Adenovirus,
• b) a stabilizing-effective amount of a non-ionic detergent of Formula I
• R—O(CH2CH2O)X—H I
• wherein
• X is 4-30, and R is a linear or branched alkyl of 10-70 carbon atoms,
• or Formula II
• 44
• wherein R is —CO2R′ having 10 to 70 carbons, and W+X+Y+Z=20, wherein R′ is as
• above for R,
• or Formula III
• 45
• wherein R is as above, and X is 5-100, Y is 25-75 and Z is 50-100,
• or Formula IV
• 46
• wherein X is 5-15 ring A is phenylene or cyclohexylene, and R′ is R as above,
• or a combination thereof, and
• c) at least one pharmaceutically acceptable carrier.

A pharmaceutical composition, comprising

• a) an airborne virus,
• b) a stabilizing-effective amount of a non-ionic detergent of according to Formula I
• R—O(CH2CH2O)X—H I
• wherein
• X is 4-30, and R is a linear or branched alkyl of 10-70 carbon atoms, or Formula II
• 47
• wherein R is —CO2R′ having 10 to 70 carbons, and W+X+Y+Z=20, wherein R′ is as above for R,
• or Formula III
• 48
• wherein R is as above, and X is 5-100, Y is 25-75 and Z is 50-100,
• or Formula IV
• 49
• wherein X is 5-15 ring A is phenylene or cyclohexylene, and R′ is R as above,
• or a combination thereof;
• and
• c) at least one pharmaceutically acceptable carrier.