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AssigneeAdvanced Cell Technology, Inc.
InventorsJose Cibelli, Michael D. West
Estimating the term…
An improved method of nuclear transfer involving the transplantation of differentiated donor cell nuclei into enucleated oocytes of a species different from the donor cell is provided. The resultant nuclear transfer units are useful for the production of isogenic embryonic stem cells, in particular human isogenic embryonic or stem cells. These embryonic or stem-like cells are useful for producing desired differentiated cells and for introduction, removal or modification, of desired genes, e.g., at specific sites of the genome of such cells by homologous recombination. These cells, which may contain a heterologous gene, are especially useful in cell transplantation therapies and for in vitro study of cell differentiation. Also, methods for improving nuclear transfer efficiency by genetically altering donor cells to inhibit apoptosis, select for a specific cell cycle and/or enhance embryonic growth and development are provided.
A method of producing embryonic or stem-like cells comprising the following steps:
A mammalian somatic cell that expresses a DNA that encodes a detectable marker, the expression of which is linked to a particular cyclin.
A method of producing human embryonic stem-like cells, comprising:
A method for producing a nuclear transfer human embryo by cross-species nuclear transfer comprising:
A method for enhancing the development of a cross-species nuclear transfer unit produced-by transferral of a human cell or nucleus into a non-human oocyte, comprising introducing into said oocyte or said human cell prior to transferral ooplasm from a rabbit oocyte.
Dependencies are inferred from the claim text.