Membrane penetrating peptides and uses thereof
OwnerYong Guo, Clarence C. Morse +2inventors
InventorsYong Guo, Clarence C. Morse, Zhengbin Yao, George A. Keesler
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Abstract
The present invention is directed to membrane penetrating peptides useful as in viv, ex vivo and in vitro intracellular delivery devices for compound of interest. More particularly, the invention involves identification of membrane penetrating peptides which may be used as protein carriers for delivery of a compound of interest to cells, to methods of delivering a compound of interest attached to membrane penetrating peptides to cells.
Claims21
A fusion protein for delivery of a compound of interest into a cell, comprising a membrane penetrating peptide attached to a compound of interest.
A method for identifying a membrane penetrating peptide, wherein a peptide comprises a sequence —(X—X—X—X)n— where n is an integer 1 to 7, and X each time is independently selected from the group consisting of arginine, histidine or lysine, by generating a conjugate peptide comprising the sequence —(X—X—X—X)n— where n is an integer 1 to 7, and X each time is independently selected from the group consisting of arginine, histidine or lysine, with a detectable protein, adding the conjugate peptide exogenously to a cell and determining if the conjugated peptide is located within the cytoplasm and/or nucleus of the cell.
A method for identifying a membrane penetrating peptide, wherein a peptide comprises a sequence derived from or overlapping with a nuclear localization sequence of a mammalian or yeast protein, by generating a conjugate peptide comprising the part or all of the nuclear localization sequence with a detectable protein, adding the conjugate peptide exogenously to a cell and determining if the conjugated peptide is located within the cytoplasm and/or nucleus of the cell.
A fusion protein for delivering a compound of interest into a cell, wherein the fusion protein comprises a membrane penetrating peptide comprising a sequence —(X—X—X—X)n— where n is an integer 1 to 7, and X each time is independently selected from the group consisting of arginine, histidine or lysine, and a compound of interest.
Dependencies are inferred from the claim text.