Polymer with superior polar retention for sample pretreatment
AssigneeVarian, Inc.
InventorsKrishna M.R. Kallury, David C. Jones, Vipul J. Shah
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Abstract
A polymeric sorbent that can be employed in the extraction and purification of polar and nonpolar molecules from a complex media (e.g. pharmaceuticals from biological matrices) by solid phase extraction (SPE). The sorbent exhibits a strong capacity for the retention of polar molecules and can facilitate the recovery of compounds possessing a range of polarities while furnishing clean extracts showing low ion suppression. The polymer is wettable and remains wetted over long periods of time.
Claims42
A polymeric sorbent comprising:
- (a) a polymeric backbone adapted to facilitate one or more interactions selected from the group consisting of a dipolar interaction and a hydrophobic interaction;
- and
- (b) an amide functionality associated with the polymeric backbone and adapted to undergo one or more interactions selected from the group consisting of proton accepting, proton donating and dipolar interactions.
A method of preparing a polymeric sorbent functionalized with an amide functionality, the method comprising:
- (a) nitrating a polymeric backbone to form a nitrated polymeric backbone;
- (b) reducing the nitrated polymeric backbone to form an aminated polymeric backbone;
- and
- (c) contacting the aminated polymeric backbone with one of an acid, an acid chloride and an acid anhydride.
A method of isolating an analyte from a sample, the method comprising:
- (a) conditioning the sorbent by washing the sorbent with an organic solvent followed by water;
- (b) contacting a sample comprising an analyte disposed in an aqueous medium with a polymeric sorbent comprising:
- (i) a polymeric backbone adapted to form at least one of a dipolar interaction and a hydrophobic interaction;
- and
- (ii) an amide functionality associated with the backbone and adapted to undergo proton accepting and proton donating interactions;
- to form a sorbent-sample complex;
- (c) washing the sorbent-sample complex with water followed by an organic solvent;
- and
- (d) eluting an analyte from the sorbent-sample complex with an eluting solvent, whereby an analyte is isolated from a sample.
Dependencies are inferred from the claim text.