Arrays
OwnerRoland Zbignieiw Kozlowski, Jonathan Michael Blackburn +2inventors
InventorsRoland Zbignieiw Kozlowski, Jonathan Michael Blackburn, Rhian Elizabeth Hayward, Grant William Wright Cameron
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Abstract
Protein arrays that allow the analysis of differentially expressed proteins in parallel are disclosed. Methods for making such arrays, and their use for screening and/or evaluating the effect of chemical entities, including drug and therapeutic moieties, are described. The arrays can also be used to screen for compounds, peptides, or proteins, which modulate the interaction between a protein and the differentially expressed protein.
Claims15
A protein array comprising a surface upon which are deposited at spatially defined locations at least two protein moieties characterised in that said protein moieties represent at least part of a set of proteins which are differentially expressed.
A method of making a protein array comprising the steps of:
- a) providing DNA coding sequences which code for two or more proteins from a set of differentially expressed proteins;
- b) expressing said coding sequences to provide individual proteins;
- c) purifying said proteins;
- and
- d) depositing said proteins at spatially defined locations on a surface to give an array.
A method for screening peptides or proteins for the ability to interact selectively with a protein on the array, said method comprising the steps of:
- (i) providing an array of differentially expressed proteins,
- (ii) contacting the array with compounds or peptides or proteins, and
- (iii)identifying the interacting partners.
A method for screening compounds or peptides or proteins for the ability to selectively modulate the interaction between a protein and a differentially expressed protein, said method comprising the steps of
- (i) providing an array of differentially expressed proteins,
- (ii) contacting the array with compounds or peptides or proteins and with other proteins thereof of interest, either simultaneously or in sequence,
- (iii) determining whether said interaction is modulated by the presence of said compounds or peptides or proteins.
Dependencies are inferred from the claim text.