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US 2003/0228692 A1Published application
Tissue decellularization
AssigneeCryolife, Inc.
InventorsSteven Goldstein, Kirby S. Black
Life of this patent
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Abstract
The present invention relates, in general, to tissue decellularization and, in particular to a method of treating tissues, for example, heart valves, tendons and ligaments, so as to render them acellular and thereby limit mineralization and/or immunoreactivity upon implementation in vivo.
Claims15
A method of producing a decellularized tissue comprising:
- i) contacting a biological tissue with a hypotonic solution under conditions such that lysis of cells of said tissue is effected, and
- ii) contacting the tissue resulting from step (i) with nuclease under conditions such that nucleic acid is degraded,
- said decellularized tissue thereby being produced.
A method of mitigating mineralization of a biological tissue transplant comprising:
- i) contacting a biological tissue with a hypotonic solution under conditions such that lysis of cells of said biological tissue is effected, and
- ii) contacting the tissue resulting from step (i) with nuclease under conditions such that nucleic acid is degraded,
- wherein the tissue resulting from step (ii) is decellularized and said mitigation is thereby effected.
A method of reducing the immunogenicity of a biological tissue transplant comprising:
- i) contacting a biological tissue with a hypotonic solution under conditions such that lysis of cells of said biological tissue is effected, and
- ii) contacting the tissue resulting from step (i) with nuclease under conditions such that nucleic acid is degraded,
- wherein the tissue resulting from step (ii) is decellularized and said reduction in immunogenicity is thereby effected.
Dependencies are inferred from the claim text.