Cell proliferation assays and methods
AssigneeRenovar Incorporated
InventorsHuaizhong Hu, Alice Puchalski
Life of this patent
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Abstract
The present invention relates to compositions and methods for conducting cell division and proliferation assays (e.g., using flow cytometry), and in particular, to improvements in the effective storage and handling of dyes and reagents used therein.
Claims28
A kit comprising:
- a container having an aliquot of CFSE dye at a concentration of from 0.8 mM to 1 mM.
The kit of claim of 1, further comprising a container having an aliquot of propidium iodide staining solution.
A kit for conducting cell proliferation assays comprising:
- a) a first container having an aliquot of first fluorescein dye, wherein said fluorescein dye comprises CFSE dye at a concentration of from 0.8 mM to 1 mM;
- and
- b) a second container having an aliquot of a second fluorescein dye.
The kit of claim of 13, further comprising a container having an aliquot of propidium iodide staining solution.
A kit comprising:
- a) a first container having an aliquot of first fluorescein dye, wherein said fluorescein dye comprises CFSE dye at a concentration of from 0.8 mM to 1 mM;
- b) a second container having an aliquot of a second fluorescein dye;
- c) two or more R-phycoerythrin conjugated antibodies;
- and
- d) instructions for using said first CFSE dye and said second fluorescein dye in a cell proliferation assay experiment.
A composition comprising a container having an aliquot of CFSE dye at a concentration of from 0.8 mM to 1 mM, wherein said aliquot of CFSE dye contains an amount of said CFSE dye suitable for a single cell proliferation assay experiment, wherein said aliquot of said CFSE dye is stable for at least 7 months.
A method of data analysis comprising:
- a) providing data from a flow cytometry assay experiment, wherein said data is displayed such that data corresponding to test cells are distinguishable from data corresponding to background cells;
- b) selecting a subset of said data from flow cytometric assay experiment such that said subset contains substantially all of said data corresponding to test cells and excludes substantially all of said data corresponding to said background cells;
- and
- c) analyzing said subset to provide a cell proliferation result.
Dependencies are inferred from the claim text.