Loading patent…
AssigneeSenomyx, Inc.
InventorsMark Zoller, Hong Xu, Lena Staszewski, Bryan Moyer, Alexy Pronin, Jon Elliot Adler, Guy Servant, Nicholas Callamaras
Estimating the term…
The present invention relates to isolated nucleic acid sequences that encode human olfactory cyclic nucleotide gated (CNG) channel subunits, and the corresponding polypeptides. The invention further relates to the use of human CNG channels to profile, screen for, and identify compounds that modulate the human olfactory CNG channel. More specifically, the invention relates to the expression of the human olfactory CNG channel in cells, preferably mammalian cells, and the use of these cells in high throughput cell-based assays to identify compounds that enhance or block human olfactory CNG function. Compounds that activate the olfactory CNG channel will enhance smell and can be used to make foods more palatable for individuals with attenuated olfactory function. Conversely, compounds that inhibit the olfactory CNG channel will inhibit smell and can be use to block malodors. Additionally, the invention relates to the use of cell-based olfactory CNG channel assays to identify modulates of G-protein coupled receptor (GPCRs) and other proteins that regulate cyclic nucleotide levels.
An isolated nucleotide sequence encoding a human OCNC1 olfactory CNG channel subunit wherein said nucleotide sequence has at least about 95% identity to SEQ ID NO:1 or encodes for a polypeptide having at least about 90% sequence identity to the polypeptide encoded by SEQ ID NO:
An isolated nucleotide sequence encoding a human OCNC2 olfactory CNG channel subunit wherein said nucleotide sequence has at least about 95% identity to SEQ ID NO:
An isolated nucleotide sequence encoding a human β1b olfactory CNG channel subunit wherein said nucleotide sequence has at least about 95% identity to SEQ ID NO:
A mammalian cell-based high throughput assay for the profiling and screening of putative modulators of a human olfactory cyclic nucleotide gated (CNG) channel comprising:
A host cell containing one or more expression vectors that encode for a polypeptide encoded by a nucleotide sequence encoding a human OCNG1 olfactory CNG channel subunit wherein said nucleotide sequence has at least 95% sequence identity to SEQ ID NO:
The method of 67 wherein said cyclic nucleotides is selected from the group consisting of cAMP or cGMP.
The method of 67 wherein said compound is an activator of adenylyl cyclase.
The method of 69 wherein said compound is forskolin.
The method of 67 wherein said compound is an activator of a G-protein.
The method of 71 wherein said compound is isoproterenol.
The method of 67 wherein said monitoring is of cation levels.
The method of 73 wherein said ions is selected from the group consisting of calcium or sodium.
Dependencies are inferred from the claim text.