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US 2003/0226752 A1Published application
Method for pH-biased isoelectric trapping separation
AssigneeGradipore Limited
InventorGyula Vigh
Life of this patent
Estimating the term…
Abstract
A method for altering the initial composition of an ampholytic component containing sample by isoelectric trapping provides for selecting a first isoelectric buffer having a pI value different from the pI value of a first ampholytic sample component in the sample, contacting the isoelectric buffer with the first ampholytic sample component, and obtaining the first ampholytic sample component in a non-isoelectric state on one side of an isoelectric separation barrier at the end of an isoelectric trapping process.
Claims63
A method of altering a composition of a sample that contains a first ampholytic component having a pI value, comprising the steps of:
- selecting an electrophoretic separation unit having an anode compartment, a separation compartment and a cathode compartment;
- selecting an anolyte having a pH value lower than the pI value of the ampholytic sample component, and introducing the anolyte into the anode compartment;
- selecting a catholyte having a pH value higher than the pI value of the ampholytic sample component, and introducing the catholyte into the cathode compartment;
- selecting an isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and different from the pI value of the ampholytic sample component;
- introducing the sample and the isoelectric buffer into the separation compartment;
- and
- trapping the ampholytic sample component in a non-isoelectric state by executing an electrophoretic separation.
4 more elements
A method of altering a composition of a sample that contains a first ampholytic component having a pI value, comprising the steps of:
- selecting an electrophoretic separation unit having an anode compartment, a first separation compartment, a second separation compartment and a cathode compartment;
- selecting an anolyte having a pH value lower than the pI value of the ampholytic sample component, and introducing the anolyte into the anode compartment;
- selecting a catholyte having a pH value higher than the pI value of the ampholytic sample component, and introducing the catholyte into the cathode compartment;
- selecting an isoelectric separation barrier having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and different from the pI value of the ampholytic sample component, wherein the isoelectric separation barrier is in contact with the first separation compartment and the second separation compartment;
- selecting an isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and different from the pI value of the ampholytic sample component and different from the pI value of the isoelectric separation barrier;
- introducing the sample into either the first or second separation compartment or both the first and second separation compartments;
- introducing the isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- and
- trapping the ampholytic sample component in a non-isoelectric state by executing an electrophoretic separation.
6 more elements
A method of altering a composition of a sample that contains a first ampholytic component having a pI value, comprising the steps of:
- selecting an electrophoretic separation unit having an anode compartment, a first separation compartment, a second separation compartment and a cathode compartment;
- selecting an anolyte having a pH value lower than the pI value of the ampholytic sample component, and introducing the anolyte into the anode compartment;
- selecting a catholyte having a pH value higher than the pI value of the ampholytic sample component, and introducing the catholyte into the cathode compartment;
- selecting an isoelectric separation barrier having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and different from the pI value of the ampholytic sample component, wherein the isoelectric separation barrier is in contact with the first separation compartment and the second separation compartment;
- selecting a first isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and different from the pI value of the ampholytic sample component and different from the pI value of the isoelectric separation barrier;
- selecting a second isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and different from the pI value of the ampholytic sample component and different from the pI value of the first isoelectric buffer and different from the pI value of the isoelectric separation barrier;
- introducing the sample into either the first or second separation compartment or both the first and second separation compartments;
- introducing the first isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- introducing the second isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- and
- trapping the ampholytic sample component in a non-isoelectric state by executing an electrophoretic separation.
8 more elements
A method of altering a composition of a sample that contains a first ampholytic component having a pI value and a second ampholytic component having a pI value, wherein the pI value of the first ampholytic component is different from the pI value of the second ampholytic component, comprising the steps of:
- selecting an electrophoretic separation unit having an anode compartment, a first separation compartment, a second separation compartment and a cathode compartment;
- selecting an anolyte having a pH value lower than the pI value of the first ampholytic sample component and lower than the pI value of the second ampholytic sample component, and introducing the anolyte into the anode compartment;
- selecting a catholyte having a pH value higher than the pI value of the first ampholytic sample component and higher than the pI value of the second ampholytic sample component, and introducing the catholyte into the cathode compartment;
- selecting an isoelectric separation barrier having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and different from the pI value of the first ampholytic sample component and different from the pI value of the second ampholytic sample component, wherein the isoelectric separation barrier is in contact with the first separation compartment and the second separation compartment;
- selecting a first isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and different from the pI value of the first ampholytic sample component and different from the pI value of the second ampholytic sample component and different from the pI value of the isoelectric separation barrier;
- selecting a second isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and different from the pI value of the first ampholytic sample component and different from the pI value of the second ampholytic sample component and different from the pI value of the first isoelectric buffer and different from the pI value of the isoelectric separation barrier;
- introducing the sample into either the first or second separation compartment or both the first and second separation compartments;
- introducing the first isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- introducing the second isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- and
- trapping the first ampholytic sample component and the second ampholytic sample component in a non-isoelectric state by executing an electrophoretic separation.
8 more elements
A method of altering a composition of a sample that contains a first ampholytic component having a pI value, comprising the steps of:
- selecting an electrophoretic separation unit having an anode compartment, a separation compartment and a cathode compartment;
- selecting an anolyte having a pH value lower than the pI value of the ampholytic sample component, and introducing the anolyte into the anode compartment;
- selecting a catholyte having a pH value higher than the pI value of the ampholytic sample component, and introducing the catholyte into the cathode compartment;
- selecting an isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pI value of the ampholytic sample component and lower than the pH value of the catholyte;
- introducing the sample and the isoelectric buffer into the separation compartment;
- and
- trapping the ampholytic sample component in a non-isoelectric state by executing an electrophoretic separation.
4 more elements
A method of altering a composition of a sample that contains a first ampholytic component having a pI value, comprising the steps of:
- selecting an electrophoretic separation unit having an anode compartment, a separation compartment and a cathode compartment;
- selecting an anolyte having a pH value lower than the pI value of the ampholytic sample component, and introducing the anolyte into the anode compartment;
- selecting a catholyte having a pH value higher than the pI value of the ampholytic sample component, and introducing the catholyte into the cathode compartment;
- selecting an isoelectric buffer having a pI value higher than the pH value of the anolyte and higher than the pI value of the ampholytic sample component and lower than the pH value of the catholyte;
- introducing the sample and the isoelectric buffer into the separation compartment;
- and
- trapping the ampholytic sample component in a non-isoelectric state by executing an electrophoretic separation.
4 more elements
A method of altering a composition of a sample that contains a first ampholytic component having a pI value, comprising the steps of:
- selecting an electrophoretic separation unit having an anode compartment, a first separation compartment, a second separation compartment and a cathode compartment;
- selecting an anolyte having a pH value lower than the pI value of the ampholytic sample component, and introducing the anolyte into the anode compartment;
- selecting a catholyte having a pH value higher than the pI value of the ampholytic sample component, and introducing the catholyte into the cathode compartment;
- selecting an isoelectric separation barrier having a pI value higher than the pH value of the anolyte and higher than the pI value of the ampholytic sample component and lower than the pH value of the catholyte, wherein the isoelectric separation barrier is in contact with the first separation compartment and the second separation compartment;
- selecting an isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pI value of the ampholytic sample component and lower than the pI value of the isoelectric separation barrier and lower than the pH value of the catholyte;
- introducing the sample into either the first or second separation compartment or both the first and second separation compartments;
- introducing the isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- and
- trapping the ampholytic sample component in a non-isoelectric state by executing an electrophoretic separation.
6 more elements
A method of altering a composition of a sample that contains a first ampholytic component having a pI value, comprising the steps of:
- selecting an electrophoretic separation unit having an anode compartment, a first separation compartment, a second separation compartment and a cathode compartment;
- selecting an anolyte having a pH value lower than the pI value of the ampholytic sample component, and introducing the anolyte into the anode compartment;
- selecting a catholyte having a pH value higher than the pI value of the ampholytic sample component, and introducing the catholyte into the cathode compartment;
- selecting an isoelectric separation barrier having a pI value higher than the pH value of the anolyte and higher than the pI value of the ampholytic sample component and lower than the pH value of the catholyte, wherein the isoelectric separation barrier is in contact with the first separation compartment and the second separation compartment;
- selecting an isoelectric buffer having a pf value higher than the pH value of the anolyte and higher than the pI value of the ampholytic sample component and lower than the pI value of the isoelectric separation barrier and lower than the pH value of the catholyte;
- introducing the sample into either the first or second separation compartment or both the first and second separation compartments;
- introducing the isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- and
- trapping the ampholytic sample component in a non-isoelectric state by executing an electrophoretic separation.
6 more elements
A method of altering a composition of a sample that contains a first ampholytic component having a pI value, comprising the steps of:
- selecting an electrophoretic separation unit having an anode compartment, a first separation compartment, a second separation compartment and a cathode compartment;
- selecting an anolyte having a pH value lower than the pI value of the ampholytic sample component, and introducing the anolyte into the anode compartment;
- selecting a catholyte having a pH value higher than the pI value of the ampholytic sample component, and introducing the catholyte into the cathode compartment;
- selecting an isoelectric separation barrier having a pI value higher than the pH value of the anolyte and lower than the pI value of the ampholytic sample component and lower than the pH value of the catholyte, wherein the isoelectric separation barrier is in contact with the first separation compartment and the second separation compartment;
- selecting an isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pI value of the isoelectric separation barrier and lower than the pI value of the ampholytic sample component and lower than the pH value of the catholyte;
- introducing the sample into either the first or second separation compartment or both the first and second separation compartments;
- introducing the isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- and
- trapping the ampholytic sample component in a non-isoelectric state by executing an electrophoretic separation.
6 more elements
A method of altering a composition of a sample that contains a first ampholytic component having a pI value, comprising the steps of:
- selecting an electrophoretic separation unit having an anode compartment, a first separation compartment, a second separation compartment and a cathode compartment;
- selecting an anolyte having a pH value lower than the pI value of the ampholytic sample component, and introducing the anolyte into the anode compartment;
- selecting a catholyte having a pH value higher than the pI value of the ampholytic sample component, and introducing the catholyte into the cathode compartment;
- selecting an isoelectric separation barrier having a pI value higher than the pH value of the anolyte and lower than the pI value of the ampholytic sample component and lower than the pH value of the catholyte, wherein the isoelectric separation barrier is in contact with the first separation compartment and the second separation compartment;
- selecting an isoelectric buffer having a pI value higher than the pH value of the anolyte and higher than the pI value of the isoelectric separation barrier and lower than the pI value of the ampholytic sample component and lower than the pH value of the catholyte;
- introducing the sample into either the first or second separation compartment or both the first and second separation compartments;
- introducing the isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- and
- trapping the ampholytic sample component in a non-isoelectric state by executing an electrophoretic separation.
6 more elements
A method of altering a composition of a sample that contains a first ampholytic component having a pI value, comprising the steps of:
- selecting an electrophoretic separation unit having an anode compartment, a first separation compartment, a second separation compartment and a cathode compartment;
- selecting an anolyte having a pH value lower than the pI value of the ampholytic sample component, and introducing the anolyte into the anode compartment;
- selecting a catholyte having a pH value higher than the pI value of the ampholytic sample component, and introducing the catholyte into the cathode compartment;
- selecting a first isoelectric separation barrier having a pI value higher than the pH value of the anolyte and lower than the pI value of the ampholytic sample component and lower than the pH value of the catholyte, wherein the isoelectric separation barrier is in contact with the first separation compartment and the second separation compartment;
- selecting an isoelectric buffer having a pI value higher than the pH value of the anolyte and higher than the pI value of the isoelectric separation barrier and higher than the pI value of the ampholytic sample component and lower than the pH value of the catholyte;
- introducing the sample into either the first or second separation compartment or both the first and second separation compartments;
- introducing the isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- and
- trapping the ampholytic sample component in a non-isoelectric state by executing an electrophoretic separation.
6 more elements
A method of altering a composition of a sample that contains a first ampholytic component having a pI value, comprising the steps of:
- selecting an electrophoretic separation unit having an anode compartment, a first separation compartment, a second separation compartment and a cathode compartment;
- selecting an anolyte having a pH value lower than the pI value of the ampholytic sample component, and introducing the anolyte into the anode compartment;
- selecting a catholyte having a pH value higher than the pI value of the ampholytic sample component, and introducing the catholyte into the cathode compartment;
- selecting an isoelectric separation barrier having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and higher than the pI value of the ampholytic sample component, wherein the isoelectric separation barrier is in contact with the first separation compartment and the second separation compartment;
- selecting a first isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and lower than the pI value of the isoelectric separation barrier and higher or lower than the pI value of the ampholytic sample component;
- selecting a second isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and lower than the pI value of the isoelectric separation barrier and higher or lower than the pI value of the ampholytic sample component and higher or lower than the pI value of the first isoelectric buffer;
- introducing the sample into either the first or second separation compartment or both the first and second separation compartments;
- introducing the first isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- introducing the second isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- and
- trapping the ampholytic sample component in a non-isoelectric state by executing an electrophoretic separation.
8 more elements
A method of altering a composition of a sample that contains a first ampholytic component having a pI value, comprising the steps of:
- selecting an electrophoretic separation unit having an anode compartment, a first separation compartment, a second separation compartment and a cathode compartment;
- selecting an anolyte having a pH value lower than the pI value of the ampholytic sample component, and introducing the anolyte into the anode compartment;
- selecting a catholyte having a pH value higher than the pI value of the ampholytic sample component, and introducing the catholyte into the cathode compartment;
- selecting an isoelectric separation barrier having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and higher than the pI value of the ampholytic sample component, wherein the isoelectric separation barrier is in contact with the first separation compartment and the second separation compartment;
- selecting a first isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and lower than the pI value of the isoelectric separation barrier and higher or lower than the pI value of the ampholytic sample component;
- selecting a second isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and higher than the pI value of the ampholytic sample component and higher than the pI value of the isoelectric separation barrier and higher than the pI value of the first isoelectric buffer;
- introducing the sample into either the first or second separation compartment or both the first and second separation compartments;
- introducing the first isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- introducing the second isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- and
- trapping the ampholytic sample component in a non-isoelectric state by executing an electrophoretic separation.
8 more elements
A method of altering a composition of a sample that contains a first ampholytic component having a pI value, comprising the steps of:
- selecting an electrophoretic separation unit having an anode compartment, a first separation compartment, a second separation compartment and a cathode compartment;
- selecting an anolyte having a pH value lower than the pI value of the ampholytic sample component, and introducing the anolyte into the anode compartment;
- selecting a catholyte having a pH value higher than the pI value of the ampholytic sample component, and introducing the catholyte into the cathode compartment;
- selecting an isoelectric separation barrier having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and higher than the pI value of the ampholytic sample component, wherein the isoelectric separation barrier is in contact with the first separation compartment and the second separation compartment;
- selecting a first isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and higher than the pI value of the isoelectric separation barrier;
- selecting a second isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and lower than the pI value of the isoelectric separation barrier and higher or lower than the pI value of the ampholytic sample component and lower than the pI value of the first isoelectric buffer;
- introducing the sample into either the first or second separation compartment or both the first and second separation compartments;
- introducing the first isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- introducing the second isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- and
- trapping the ampholytic sample component in a non-isoelectric state by executing an electrophoretic separation.
8 more elements
A method of altering a composition of a sample that contains a first ampholytic component having a pI value, comprising the steps of:
- selecting an electrophoretic separation unit having an anode compartment, a first separation compartment, a second separation compartment and a cathode compartment;
- selecting an anolyte having a pH value lower than the pI value of the ampholytic sample component, and introducing the anolyte into the anode compartment;
- selecting a catholyte having a pH value higher than the pI value of the ampholytic sample component, and introducing the catholyte into the cathode compartment;
- selecting an isoelectric separation barrier having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and higher than the pI value of the ampholytic sample component, wherein the isoelectric separation barrier is in contact with the first separation compartment and the second separation compartment;
- selecting a first isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and higher than the pI value of the ampholytic sample component and higher than the pI value of the isoelectric separation barrier;
- selecting a second isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and higher than the pI value of the ampholytic sample component and higher than the pI value of the isoelectric separation barrier and lower or higher than the pI value of the first isoelectric buffer;
- introducing the sample into either the first or second separation compartment or both the first and second separation compartments;
- introducing the first isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- introducing the second isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- and
- trapping the ampholytic sample component in a non-isoelectric state by executing an electrophoretic separation.
8 more elements
A method of altering a composition of a sample that contains a first ampholytic component having a pI value, comprising the steps of:
- selecting an electrophoretic separation unit having an anode compartment, a first separation compartment, a second separation compartment and a cathode compartment;
- selecting an anolyte having a pH value lower than the pI value of the ampholytic sample component, and introducing the anolyte into the anode compartment;
- selecting a catholyte having a pH value higher than the pI value of the ampholytic sample component, and introducing the catholyte into the cathode compartment;
- selecting an isoelectric separation barrier having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and lower than the pI value of the ampholytic sample component, wherein the isoelectric separation barrier is in contact with the first separation compartment and the second separation compartment;
- selecting a first isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and lower than the pI value of the ampholytic sample component and lower than the pI value of the isoelectric separation barrier;
- selecting a second isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and lower than the pI value of the ampholytic sample component and lower than the pI value of the isoelectric separation barrier and lower or higher than the pI value of the first isoelectric buffer;
- introducing the sample into either the first or second separation compartment or both the first and second separation compartments;
- introducing the first isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- introducing the second isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- and
- trapping the ampholytic sample component in a non-isoelectric state by executing an electrophoretic separation.
8 more elements
A method of altering a composition of a sample that contains a first ampholytic component having a pI value, comprising the steps of:
- selecting an electrophoretic separation unit having an anode compartment, a first separation compartment, a second separation compartment and a cathode compartment;
- selecting an anolyte having a pH value lower than the pI value of the ampholytic sample component, and introducing the anolyte into the anode compartment;
- selecting a catholyte having a pH value higher than the pI value of the ampholytic sample component, and introducing the catholyte into the cathode compartment;
- selecting an isoelectric separation barrier having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and lower than the pI value of the ampholytic sample component, wherein the isoelectric separation barrier is in contact with the first separation compartment and the second separation compartment;
- selecting a first isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and higher than the pI value of the isoelectric separation barrier and lower or higher than the pI value of the ampholytic sample component;
- selecting a second isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and lower than the pI value of the ampholytic sample component and lower than the pI value of the isoelectric separation barrier and lower than the pI value of the first isoelectric buffer;
- introducing the sample into either the first or second separation compartment or both the first and second separation compartments;
- introducing the first isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- introducing the second isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- and
- trapping the ampholytic sample component in a non-isoelectric state by executing an electrophoretic separation.
8 more elements
A method of altering a composition of a sample that contains a first ampholytic component having a pI value, comprising the steps of:
- selecting an electrophoretic separation unit having an anode compartment, a first separation compartment, a second separation compartment and a cathode compartment;
- selecting an anolyte having a pH value lower than the pI value of the ampholytic sample component, and introducing the anolyte into the anode compartment;
- selecting a catholyte having a pH value higher than the pI value of the ampholytic sample component, and introducing the catholyte into the cathode compartment;
- selecting an isoelectric separation barrier having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and lower than the pI value of the ampholytic sample component, wherein the isoelectric separation barrier is in contact with the first separation compartment and the second separation compartment;
- selecting a first isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and lower than the pI value of the isoelectric separation barrier and lower than the pI value of the ampholytic sample component;
- selecting a second isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and higher than the pI value of the isoelectric separation barrier and higher than the pI value of the first isoelectric buffer and higher or lower than the pI value of the ampholytic sample component;
- introducing the sample into either the first or second separation compartment or both the first and second separation compartments;
- introducing the first isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- introducing the second isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- and
- trapping the ampholytic sample component in a non-isoelectric state by executing an electrophoretic separation.
8 more elements
A method of altering a composition of a sample that contains a first ampholytic component having a pI value, comprising the steps of:
- selecting an electrophoretic separation unit having an anode compartment, a first separation compartment, a second separation compartment and a cathode compartment;
- selecting an anolyte having a pH value lower than the pI value of the ampholytic sample component, and introducing the anolyte into the anode compartment;
- selecting a catholyte having a pH value higher than the pI value of the ampholytic sample component, and introducing the catholyte into the cathode compartment;
- selecting an isoelectric separation barrier having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and lower than the pI value of the ampholytic sample component, wherein the isoelectric separation barrier is in contact with the first separation compartment and the second separation compartment;
- selecting a first isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and higher than the pI value of the isoelectric separation barrier and higher or lower than the pI value of the ampholytic sample component;
- selecting a second isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and higher than the pI value of the isoelectric separation barrier and higher or lower than the pI value of the ampholytic sample component and lower or higher than the pI value of the first isoelectric buffer;
- introducing the sample into either the first or second separation compartment or both the first and second separation compartments;
- introducing the first isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- introducing the second isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- and
- trapping the ampholytic sample component in a non-isoelectric state by executing an electrophoretic separation.
8 more elements
A method of altering a composition of a sample that contains a first ampholytic component having a pI value and a second ampholytic component having a pI value, comprising the steps of:
- selecting an electrophoretic separation unit having an anode compartment, a first separation compartment, a second separation compartment and a cathode compartment;
- selecting an anolyte having a pH value lower than the pI value of the first ampholytic sample component and lower than the pI value of the second ampholytic sample component, and introducing the anolyte into the anode compartment;
- selecting a catholyte having a pH value higher than the pI value of the first ampholytic sample component and higher than the pI value of the second ampholytic sample component, and introducing the catholyte into the cathode compartment;
- selecting an isoelectric separation barrier having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and higher than the pI value of the first ampholytic sample component and higher than the pI value of the second ampholytic sample component, wherein the isoelectric separation barrier is in contact with the first separation compartment and the second separation compartment;
- selecting a first isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and lower than the pI value of the isoelectric separation barrier and higher or lower than the pI value of the first ampholytic sample component and higher or lower than the pI value of the second ampholytic sample component;
- selecting a second isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and lower than the pI value of the isoelectric separation barrier and higher or lower than the pI value of the first ampholytic sample component and higher or lower than the pI value of the second ampholytic sample component and higher or lower than the pI value of the first isoelectric buffer;
- introducing the sample into either the first or second separation compartment or both the first and second separation compartments;
- introducing the first isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- introducing the second isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- and
- trapping the ampholytic sample component in a non-isoelectric state by executing an electrophoretic separation.
8 more elements
A method of altering a composition of a sample that contains a first ampholytic component having a pI value and a second ampholytic component having a pI value, comprising the steps of:
- selecting an electrophoretic separation unit having an anode compartment, a first separation compartment, a second separation compartment and a cathode compartment;
- selecting an anolyte having a pH value lower than the pI value of the first ampholytic sample component and lower than the pI value of the second ampholytic sample component, and introducing the anolyte into the anode compartment;
- selecting a catholyte having a pH value higher than the pI value of the first ampholytic sample component and higher than the pI value of the second ampholytic sample component, and introducing the catholyte into the cathode compartment;
- selecting an isoelectric separation barrier having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and lower than the pI value of the first ampholytic sample component and lower than the pI value of the second ampholytic sample component, wherein the isoelectric separation barrier is in contact with the first separation compartment and the second separation compartment;
- selecting a first isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and higher than the pI value of the isoelectric separation barrier and higher or lower than the pI value of the first ampholytic sample component and higher or lower than the pI value of the second ampholytic sample component;
- selecting a second isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and lower than the pI value of the isoelectric separation barrier and higher or lower than the pI value of the first ampholytic sample component and higher or lower than the pI value of the second ampholytic sample component and higher or lower than the pI value of the first isoelectric buffer;
- introducing the sample into either the first or second separation compartment or both the first and second separation compartments;
- introducing the first isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- introducing the second isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- and
- trapping the first ampholytic sample component and the second ampholytic sample component in a non-isoelectric state by executing an electrophoretic separation.
8 more elements
A method of altering a composition of a sample that contains a first ampholytic component having a pI value and a second ampholytic component having a pI value, comprising the steps of:
- selecting an electrophoretic separation unit having an anode compartment, a first separation compartment, a second separation compartment and a cathode compartment;
- selecting an anolyte having a pH value lower than the pI value of the first ampholytic sample component and lower than the pI value of the second ampholytic sample component, and introducing the anolyte into the anode compartment;
- selecting a catholyte having a pH value higher than the pI value of the first ampholytic sample component and higher than the pI value of the second ampholytic sample component, and introducing the catholyte into the cathode compartment;
- selecting an isoelectric separation barrier having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and higher than the pI value of the first ampholytic sample component and lower than the pI value of the second ampholytic sample component, wherein the isoelectric separation barrier is in contact with the first separation compartment and the second separation compartment;
- selecting a first isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and lower than the pI value of the isoelectric separation barrier and higher or lower than the pI value of the first ampholytic sample component and lower than the pI value of the second ampholytic sample component;
- selecting a second isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and higher than the pI value of the isoelectric separation barrier and higher than the pI value of the first ampholytic sample component and higher or lower than the pI value of the second ampholytic sample component and higher than the pI value of the first isoelectric buffer;
- introducing the sample into either the first or second separation compartment or both the first and second separation compartments;
- introducing the first isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- introducing the second isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- and
- trapping the first ampholytic sample component and the second ampholytic sample component in a non-isoelectric state by executing an electrophoretic separation.
8 more elements
A method of altering a composition of a sample that contains a first ampholytic component having a pI value and a second ampholytic component having a pI value, comprising the steps of:
- selecting an electrophoretic separation unit having an anode compartment, a first separation compartment, a second separation compartment and a cathode compartment;
- selecting an anolyte having a pH value lower than the pI value of the first ampholytic sample component and lower than the pI value of the second ampholytic sample component, and introducing the anolyte into the anode compartment;
- selecting a catholyte having a pH value higher than the pI value of the first ampholytic sample component and higher than the pI value of the second ampholytic sample component, and introducing the catholyte into the cathode compartment;
- selecting an isoelectric separation barrier having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and higher than the pI value of the first ampholytic sample component and lower than the pI value of the second ampholytic sample component, wherein the isoelectric separation barrier is in contact with the first separation compartment and the second separation compartment;
- selecting a first isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and higher than the pI value of the isoelectric separation barrier and higher than the pI value of the first ampholytic sample component and higher or lower than the pI value of the second ampholytic sample component;
- selecting a second isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and lower than the pI value of the isoelectric separation barrier and higher or lower than the pI value of the first ampholytic sample component and lower than the pI value of the second ampholytic sample component and lower than the pI value of the first isoelectric buffer;
- introducing the sample into either the first or second separation compartment or both the first and second separation compartments;
- introducing the first isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- introducing the second isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- and
- trapping the first ampholytic sample component and the second ampholytic sample component in a non-isoelectric state by executing an electrophoretic separation.
8 more elements
A method of altering a composition of a sample that contains a first ampholytic component having a pI value and a second ampholytic component having a pI value, comprising the steps of:
- selecting an electrophoretic separation unit having an anode compartment, a first separation compartment, a second separation compartment and a cathode compartment;
- selecting an anolyte having a pH value lower than the pI value of the first ampholytic sample component and lower than the pI value of the second ampholytic sample component, and introducing the anolyte into the anode compartment;
- selecting a catholyte having a pH value higher than the pI value of the first ampholytic sample component and higher than the pI value of the second ampholytic sample component, and introducing the catholyte into the cathode compartment;
- selecting an isoelectric separation barrier having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and lower than the pI value of the first ampholytic sample component and higher than the pI value of the second ampholytic sample component, wherein the isoelectric separation barrier is in contact with the first separation compartment and the second separation compartment;
- selecting a first isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and lower than the pI value of the isoelectric separation barrier and lower than the pI value of the first ampholytic sample component and higher or lower than the pI value of the second ampholytic sample component;
- selecting a second isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and higher than the pI value of the isoelectric separation barrier and higher or lower than the pI value of the first ampholytic sample component and higher than the pI value of the second ampholytic sample component and higher than the pI value of the first isoelectric buffer;
- introducing the sample into either the first or second separation compartment or both the first and second separation compartments;
- introducing the first isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- introducing the second isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- and
- trapping the first ampholytic sample component and the second ampholytic sample component in a non-isoelectric state by executing an electrophoretic separation.
8 more elements
A method of altering a composition of a sample that contains a first ampholytic component having a pI value and a second ampholytic component having a pI value, comprising the steps of:
- selecting an electrophoretic separation unit having an anode compartment, a first separation compartment, a second separation compartment and a cathode compartment;
- selecting an anolyte having a pH value lower than the pI value of the first ampholytic sample component and lower than the pI value of the second ampholytic sample component, and introducing the anolyte into the anode compartment;
- selecting a catholyte having a pH value higher than the pI value of the first ampholytic sample component and higher than the pI value of the second ampholytic sample component, and introducing the catholyte into the cathode compartment;
- selecting an isoelectric separation barrier having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and lower than the pI value of the first ampholytic sample component and higher than the pI value of the second ampholytic sample component, wherein the isoelectric separation barrier is in contact with the first separation compartment and the second separation compartment;
- selecting a first isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and higher than the pI value of the isoelectric separation barrier and higher or lower than the pI value of the first ampholytic sample component and higher than the pI value of the second ampholytic sample component;
- selecting a second isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and lower than the pI value of the isoelectric separation barrier and lower than the pI value of the first ampholytic sample component and higher or lower than the pI value of the second ampholytic sample component and lower than the pI value of the first isoelectric buffer;
- introducing the sample into either the first or second separation compartment or both the first and second separation compartments;
- introducing the first isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- introducing the second isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- and
- trapping the first ampholytic sample component and the second ampholytic sample component in a non-isoelectric state by executing an electrophoretic separation.
8 more elements
A method of altering a composition of a sample that contains a first ampholytic component having a pI value and a second ampholytic component having a pI value, comprising the steps of:
- selecting an electrophoretic separation unit having an anode compartment, a first separation compartment, a second separation compartment and a cathode compartment;
- selecting an anolyte having a pH value lower than the pI value of the first ampholytic sample component and lower than the pI value of the second ampholytic sample component, and introducing the anolyte into the anode compartment;
- selecting a catholyte having a pH value higher than the pI value of the first ampholytic sample component and higher than the pI value of the second ampholytic sample component, and introducing the catholyte into the cathode compartment;
- selecting an isoelectric separation barrier having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and higher than the pI value of the first ampholytic sample component and lower than the pI value of the second ampholytic sample component, wherein the isoelectric separation barrier is in contact with the first separation compartment and the second separation compartment;
- selecting a first isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and higher than the pI value of the isoelectric separation barrier and higher than the pI value of the first ampholytic sample component and higher or lower than the pI value of the second ampholytic sample component;
- selecting a second isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and higher than the pI value of the isoelectric separation barrier and higher than the pI value of the first ampholytic sample component and higher or lower than the pI value of the first ampholytic sample component and higher or lower than the pI value of the first isoelectric buffer;
- introducing the sample into either the first or second separation compartment or both the first and second separation compartments;
- introducing the first isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- introducing the second isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- and
- trapping the first ampholytic sample component and the second ampholytic sample component in a non-isoelectric state by executing an electrophoretic separation.
8 more elements
A method of altering a composition of a sample that contains a first ampholytic component having a pI value and a second ampholytic component having a pI value, comprising the steps of:
- selecting an electrophoretic separation unit having an anode compartment, a first separation compartment, a second separation compartment and a cathode compartment;
- selecting an anolyte having a pH value lower than the pI value of the first ampholytic sample component and lower than the pI value of the second ampholytic sample component, and introducing the anolyte into the anode compartment;
- selecting a catholyte having a pH value higher than the pI value of the first ampholytic sample component and higher than the pI value of the second ampholytic sample component, and introducing the catholyte into the cathode compartment;
- selecting an isoelectric separation barrier having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and lower than the pI value of the first ampholytic sample component and higher than the pI value of the second ampholytic sample component, wherein the isoelectric separation barrier is in contact with the first separation compartment and the second separation compartment;
- selecting a first isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and higher than the pI value of the isoelectric separation barrier and higher or lower than the pI value of the first ampholytic sample component and higher than the pI value of the second ampholytic sample component;
- selecting a second isoelectric buffer having a pI value higher than the pH value of the anolyte and lower than the pH value of the catholyte and higher than the pI value of the isoelectric separation barrier and higher or lower than the pI value of the first ampholytic sample component and higher than the pI value of the second ampholytic sample component and higher or lower than the pI value of the second isoelectric buffer;
- introducing the sample into either the first or second separation compartment or both the first and second separation compartments;
- introducing the first isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- introducing the second isoelectric buffer into either the first or second separation compartment or both the first and second separation compartments;
- and
- trapping the first ampholytic sample component and the second ampholytic sample component in a non-isoelectric state by executing an electrophoretic separation.
8 more elements
Dependencies are inferred from the claim text.