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US 2003/0226162 A1Published application
Transgenic cells expressing glucosyltransferase nucleic acids
AssigneeThe University of York
InventorsDiana Joy Bowles, Yi Li, Eng-Kiat Lim
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Abstract
The invention relates to transgenic cells which have been transformed with nucleic acids encoding glucosyltransferase polypeptides (GTases) and vectors for use in transformation of said cells.
Claims18
A transgenic plant comprising a nucleic acid molecule which encodes a polypeptide which:
- i) has glucosyltransferase activity;
- ii) is encoded by a nucleic acid molecule selected from the group comprising sequences presented in FIGS. 1A, 2A, 3A, 4A, 5A, 6A, 7A, 8A, 9A, 10A, 11A, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32;
- iii) nucleic acids which hybridise to the sequences represented in (ii) above and which modify biosynthetic intermediates in lignin polymerization;
- and
- iv) nucleic acid sequences which are degenerate as a result of the genetic code to the sequences defined in (ii) and (iii) above.
2 more elements
A transgenic eukaryotic cell comprising a nucleic acid molecule which encodes a polypeptide which:
- i) has glucosyltransferase activity;
- ii) is encoded by a nucleic acid molecule selected from the group comprising of sequences of FIGS. 1A, 2A, 3A, 4A, 5A, 6A, 7A, 8A, 9A, 10A, 11A, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32;
- iii) nucleic acids which hybridise to the sequences represented in (ii) above and which glucosylate an anti-oxidant selected from the group consisting of quercetin;
- luteolin;
- catechin;
- epicatechin;
- cyanidin;
- caffeic acid;
- and
- iv) nucleic acid sequences which are degenerate as a result of the genetic code to the sequences defined in (ii) and (iii) above.
7 more elements
A transgenic prokaryotic cell comprising a nucleic acid molecule which encodes a polypeptide which:
- i) has glucosyltransferase activity;
- ii) is encoded by a nucleic acid molecule selected from the group comprising sequences of FIGS. 1A, 2A, 3A, 4A, 5A, 6A, 7A, 8A, 9A, 10A, 11A, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32;
- iii) nucleic acids which hybridism to the sequences represented in (ii) above which glucosylate an anti-oxidant selected from the group consisting of quercetin;
- luteolin;
- catechin;
- epicatechin;
- cyanidin;
- caffeic acid;
- and
- iv) nucleic acid sequences which are degenerate as a result of the genetic code to the sequences defined in (ii) and (iii) above.
7 more elements
Dependencies are inferred from the claim text.