Secondary structure defining database and methods for determining identity and geographic origin of an unknown bioagent for environmental testing thereby

A service providing information related to a bioagent in an environmental sample comprising:

• providing a dimensional master database for storing a molecular mass, an identity and a detail corresponding to a plurality of known bioagents and, said master database storing the molecular mass, the identity and the detail for a plurality of known bioagents;
• interrogating the master database with an identification request of an unknown bioagent in said environmental sample to generate a response;
• and
• delivering said response from the master database to a requester.

A method of determining a geographical origin of a selected bioagent in an environmental sample using a database of molecular masses of known bioagents comprising:

• contacting nucleic acid from said selected bioagent in said environmental sample with at least one pair of oligonucleotide primers which hybridize to sequences of said nucleic acid, wherein said sequences flank a variable nucleic acid sequence of the bioagent in said environmental sample;
• producing an amplification product of said variable nucleic acid sequence;
• determining a first molecular mass of said amplification product;
• and
• comparing said first molecular mass to the molecular masses of known bioagents for determining a geographic origin of said selected bioagent, said comparison determining an identity and a geographic origin of said selected bioagent in said environmental sample.