Novel vectors and genes exhibiting increased expression
OwnerCharles R. Ill, Jose E.N. Gonzales +2inventors
InventorsCharles R. Ill, Jose E.N. Gonzales, Claire Q. Yang, Scott Bidlingmaier
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Abstract
Disclosed is a liver specific expression vector designed for expression of blood coagulation factor proteins. The expression vector comprises a DNA coding sequence for a blood coagulation factor operably linked to a liver-specific promoter and a liver-specific enhancer, wherein the promoter and enhancer are derived from different genes. In a particular embodiment, the liver-specific promoter is the human thyroid binding globulin promoter and the liver-specific enhancer is the alpha-1 microglobulin/bikunin enhancer. The expression vector may further contain modifications for optimal liver-specific expression.
Claims26
An expression vector comprising a DNA sequence encoding a blood coagulation factor operably linked to a liver-specific promoter and a liver-specific enhancer, wherein the promoter and enhancer are derived from different genes, and wherein the liver-specific promoter is the human thyroid binding globulin promoter.
An expression vector comprising the human thyroid binding globulin promoter and the alpha-1 microglobulin/bikunin enhancer, wherein the human promoter and enhancer are located upstream of a DNA sequence encoding a human Factor VIII protein.
An expression vector comprising a liver-specific promoter and a liver-specific enhancer, wherein said promoter and enhancer are derived from different genes and are located upstream from a DNA sequence encoding a human Factor VIII protein.
Dependencies are inferred from the claim text.