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OwnerDirk Franke, Georg Sprenger +6inventors
InventorsDirk Franke, Georg Sprenger, Ralf Takors, Michael Muller, Markus Halfar, Rolg Muller, Jorg Thommes, Volke Lorbach
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In the production of genetically-modified, non-pathogenic organisms particularly an improved production and isolation of trans-dihydroxycyclohexadiene carboxylic acids and/or derivatives thereof, the synthesis of the transhydroxy cyclohexadiene carboxylic acids is performed in an enantiomer-pure form without detectable impurities with chorismate by cultivation of at least one genetically altered non-pathogenic organism which has an aromate embolism and has, in comparison with a corresponding, genetically not altered organism, an increased activity of the isochorismate synthase (EntC) and isochorismatase (EntB) and an activity of the 2,3 dihydroxybenzoate synthase (EntA) which is close to zero, or an increased activity of the isochorismatase (EntB) and a reduced activity of the isochorismate synthase (EntC) and an activity of the 2,3-dihydroxybenzoat-synthase (entA) of close to zero.
A method for the improved production of transdihydroxycyclohexadiene carboxylic acid and derivative products thereof, comprising the steps of performing the synthesis of the trans-hydroxycyclohexadiene carboxylic acids in an enantiomer-pure form without detectable impurities with chorismate on the basis of renewable carbon sources by cultivation of at least one genetically altered non-pathogenic organism which has an aromate embolism and has, in comparison with a corresponding, genetically not altered organism, one of
A genetically altered organism for the improved production of trans-dihydroxycylohexadiene carboxylic acids in enantiomer pure form without detectable impurities by chorismate, said genetically changed organism being
Dependencies are inferred from the claim text.