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OwnerRobert S. Molday, Jinhi Ahn +1inventors
InventorsRobert S. Molday, Jinhi Ahn, William S. Hauswirth
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The present invention provides a system and method for expressing a functional ABC (ATP-binding cassette) transporter in a host cell. A system comprises two or more expression vectors each comprising a nucleic acid molecule encoding one or more domains of the ABCR transporter gene and a means for expressing the nucleic acid molecule. Each expression vector of the system includes a nucleic acid molecule that encodes a domain that is functionally complementary to domains contained in the other expression vectors of the system but when taken together comprise the full ABCR transporter gene. Co-transfection of the expression vectors into a host cell provides co-expression of each of the domains of the protein which assemble to form an ABC transporter protein having functional characteristics of the full-length protein.
A nucleic acid composition for expression of a functional ABCR transporter in a host cell, said nucleic acid composition comprising two or more different nucleic acid molecules, each nucleic acid molecule encoding one or more domains of an ABCR transporter, wherein said at least one of the domains encoded by each nucleic acid molecule are functionally complementary.
A system for expressing an ABCR transporter in a host cell comprising two or more expression vectors, each expression vector comprising a different nucleic acid molecule and each nucleic acid molecule encoding one or more domains of an ABCR transporter, wherein said at least one of the domains encoded by each nucleic acid molecule is a functionally complementary domain, and
A method for expressing an ABCR transporter in a host cell comprising:
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