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US 2003/0224466 A1Published application
Neuroblastoma cell lines expressing the alpha2delta subunit of calcium channels and methods therefore
OwnerSui-Po Zhang, Susan K. Yagel +1inventors
InventorsSui-Po Zhang, Susan K. Yagel, Ellen E. Codd
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Abstract
The present invention provides methods for identifying test substances that bind to the α2δ subunit of a calcium channel.
Claims11
A method for detecting binding of a test substance to an α2δ subunit of a calcium channel comprising the steps of:
- (a) contacting a neuroblastoma cell membrane sample comprising the α2δ subunit of a calcium channel with gabapentin and a test substance;
- (b) detecting binding of the gabapentin to the cell membrane;
- and
- (c) comparing the level of binding of gabapentin as compared with a control sample lacking the test substance.
1 more element
A test substance identified by a method comprising the steps of:
- (a) contacting a neuroblastoma cell membrane sample comprising the α2δ subunit of a calcium channel with gabapentin and a test substance;
- (b) detecting binding of the gabapentin to the cell membrane;
- and
- (c) comparing the level of binding of gabapentin as compared with a control sample lacking the test substance.
1 more element
A method for identifying a test substance capable of binding to an α2δ subunit of a calcium channel comprising the steps of:
- (a) incubating an IMR32 cell membrane with radioactive gabapentin (GBP) and a test substance, wherein the membrane comprises an α2δ subunit of calcium channel and where the contact is for sufficient time to allow GBP binding to the α2δ subunit of calcium channels in the cell membranes;
- (b) separating the cell membranes from unbound radioactive GBP;
- (c) measuring binding of the radioactive GBP to the cell membranes;
- and
- (d) identifying a compound that inhibits GBP binding by a reduction of the amount of radioactive GBP in step (c) to an established control.
2 more elements
Dependencies are inferred from the claim text.