Methods and compositions for use in preparing siRNAs
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsJason Myers, James Ferrell
Life of this patent
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Abstract
Methods and compositions for producing siRNAs, e.g., in the form of a d-siRNA composition, from dsRNAs are provided. In the subject methods, a dsRNA is contacted with a composition that includes an activity that cleaves dsRNA into siRNAs, where the composition efficiently cleaves dsRNA into siRNAs. siRNAs produced by the subject methods find use in a variety of applications, particularly in applications where the specific reduction or silencing of a gene is desired. Also provided are kits for use in practicing the subject invention.
Claims21
A method for producing siRNA from an initial dsRNA, said method comprising:
- contacting said dsRNA with a composition enriched for a protein having an activity that cleaves a dsRNA substrate into fragments having siRNA activity to produce said siRNA, wherein said composition efficiently produces siRNAs from dsRNA.
A kit for use in preparing siRNA from dsRNA, said kit comprising:
- a composition enriched for a protein having an activity that cleaves a dsRNA substrate into fragments having siRNA activity to produce said siRNA, wherein said composition efficiently produces siRNAs from dsRNA.
Dependencies are inferred from the claim text.