Human G-protein chemokine receptor HSATU68
AssigneeHuman Genome Sciences, Inc.
InventorYi Li
Life of this patent
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Abstract
Human G-protein chemokine receptor polypeptides and DNA (RNA) encoding such polypeptides and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such polypeptides for identifying antagonists and agonists to such polypeptides and methods of using the agonists and antagonists therapeutically to treat conditions related to the underexpression and overexpression of the G-protein chemokine receptor polypeptides, respectively. Also disclosed are diagnostic methods for detecting a mutation in the G-protein chemokine receptor nucleic acid sequences and detecting a level of the soluble form of the receptors in a sample derived from a host.
Claims24
An isolated nucleic acid molecule comprising a polynucleotide having a nucleotide sequence at least 95% identical to a sequence selected from the group consisting of:
- (a) a polynucleotide fragment of SEQ ID NO:
- 1, SEQ ID NO:
- 3, or a polynucleotide fragment of the cDNA sequence contained in ATCC Deposit NO:
- 97334, which is hybridizable to SEQ ID NO:
- 1 or SEQ ID NO:
- 3;
- (b) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:
- 2, SEQ ID NO:
- 4, or a polypeptide fragment encoded by the cDNA sequence contained in cDNA ATCC Deposit NO:
- 97334, which is hybridizable to SEQ ID NO:
- 1 or SEQ ID NO:
- 3;
- (c) a polynucleotide encoding a polypeptide fragment of a polypeptide encoded by SEQ ID NO:
- 1, SEQ ID NO:
- 3, or a polypeptide fragment encoded by the cDNA sequence contained in cDNA ATCC Deposit NO:
- 97334, which is hybridizable to SEQ ID NO:
- 1 or SEQ ID NO:
- 3;
- (d) a polynucleotide encoding a polypeptide domain of SEQ ID NO:
- 2, SEQ ID NO:
- 4, or a polypeptide domain encoded by the cDNA sequence contained in cDNA ATCC Deposit NO:
- 97334, which is hybridizable to SEQ ID NO:
- 1 or SEQ ID NO:
- 3;
- (e) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:
- 2, SEQ ID NO:
- 4, or a polypeptide epitope encoded by the cDNA sequence contained in cDNA ATCC Deposit NO:
- 97334, which is hybridizable to SEQ ID NO:
- 1 or SEQ ID NO:
- 3;
- (f) a polynucleotide encoding a polypeptide of SEQ ID NO:
- 2, SEQ ID NO:
- 4, or the cDNA sequence contained in cDNA ATCC Deposit NO:
- 97334, which is hybridizable to SEQ ID NO:
- I or SEQ ID NO:
- 3, having biological activity;
- (g) a polynucleotide which is a variant of SEQ ID NO:
- 1 or SEQ ID NO:
- 3;
- (h) a polynucleotide which is an allelic variant of SEQ ID NO:
- 1 or SEQ ID NO:
- 3;
- (i) a polynucleotide which encodes a species homologue of the SEQ ID NO:
- 2 or SEQ ID NO:
- 4;
- (j) a polynucleotide capable of hybridizing under stringent conditions to any one of the polynucleotides specified in (a)-(i), wherein said polynucleotide does not hybridize under stringent conditions to a nucleic acid molecule having a nucleotide sequence of only A residues or of only T residues.
An isolated polypeptide comprising an amino acid sequence at least 90% identical to a sequence selected from the group consisting of:
- (a) a polypeptide fragment of SEQ ID NO:
- 2, SEQ ID NO:
- 4, or the polypeptide encoded by the cDNA contained in ATCC Deposit NO:
- 97334;
- (b) a polypeptide fragment of SEQ ID NO:
- 2, SEQ ID NO:
- 4, or the polypeptide encoded by the cDNA contained in ATCC Deposit NO:
- 97334, having biological activity;
- (c) a polypeptide domain of SEQ ID NO:
- 2, SEQ ID NO:
- 4, or the polypeptide encoded by the cDNA contained in ATCC Deposit NO:
- 97334;
- (d) a polypeptide epitope of SEQ ID NO:
- 2, SEQ ID NO:
- 4, or the polypeptide encoded by the cDNA contained in ATCC Deposit NO:
- 97334;
- (e) a full length protein of SEQ ID NO:
- 2, SEQ ID NO:
- 4, or the polypeptide encoded by the cDNA contained in ATCC Deposit NO:
- 97334;
- (f) a variant of SEQ ID NO:
- 2 or SEQ ID NO:
- 4;
- (g) an allelic variant of SEQ ID NO:
- 2 or SEQ ID NO:
- 4;
- or
- (h) a species homologue of the SEQ ID NO:
- 2 or SEQ ID NO:
- 4.
The gene corresponding to the cDNA sequence of SEQ ID NO:
- 2 or SEQ ID NO:
- 4.
A method of identifying an activity in a biological assay, wherein the method comprises:
- (a) expressing SEQ ID NO:
- 1 or SEQ ID NO:
- 3 in a cell;
- (b) isolating the supernatant;
- (c) detecting an activity in a biological assay;
- and
- (d) identifying the protein in the supernatant having the activity.
Dependencies are inferred from the claim text.