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OwnerCarl Borrebaeck, Anki Malmborg +1inventors
InventorsCarl Borrebaeck, Anki Malmborg, Eskil Soderlind
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A method for selecting nucleic acid sequences encoding ligand and receptor molecules capable of specific binding to each other is disclosed in which nucleic acid encoding ligand or receptor molecules is expressed in a host microorganism in combination with a surface molecule, such as E. coli pili, so that the ligand or receptor are displayed on the surface of the host microorganism. A replicable genetic unit, such as a filamentous bacteriophage, is used to display candidate binding partners to the ligand or receptor, with the binding of the ligand or receptor to the candidate binding partner mediating the transfer of nucleic acid from the replicable genetic unit to the microorganism. The method can be highly selective as the host microorganism is modified so that it does not display the surface molecule other than as a fusion with the ligand receptor molecule. The method is rapid and simple and opens up new applications based on the detection of ligand and receptors where both are unknown.
A a method for selecting nucleic acid sequences encoding ligand and receptor molecules capable of specific binding to each other, the method comprising:
A method for selecting specific ligand and receptor encoding sequences wherein:
The method of any one of the preceding claims wherein the method is used to screen a library of microorganisms displaying different ligand or receptor molecules against libraries of replicable genetic units displaying candidate specific binding partners for the ligand or receptor molecules.
The method of any one of the preceding claims wherein the surface molecule encoding sequence of step (a) is combined with a receptor encoding sequence, or a functional fragment thereof, and the surface molecule encoding sequence of step (b) is fused to a ligand encoding sequence, or a functional fragment thereof.
The method of any one of the preceding claims wherein the host microorganism is modified to display the ligand or receptor by a bacterial display vector.
The method of any one of the preceding claims wherein the host microorganism is the bacteria E. coli.
The method of any one of the preceding claims wherein the surface molecule of the host organism is selected from the group OmpA, IgA protease or pilin.
The method of any one of the preceding claims wherein the replicable genetic unit is a phage display vector.
The method of any one of the preceding claims wherein the surface molecule of the replicable genetic unit is pIII.
The method of any one of the preceding claims wherein the ligand is an antigen and the receptor is an antibody.
A kit for use in a method for selecting nucleic acid sequences encoding ligand and receptor molecules capable of specific binding to each other, the kit comprising:
Dependencies are inferred from the claim text.