Proteins for helicobacter diagnosis
AssigneeThe Regents of the University of California
InventorsStephen Barthold, Sunlian Feng, Emir Hodzic
Life of this patent
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Abstract
The present invention provides isolated polynucleotides encoding the Helicobacter bilis antigens P167 and P17Hb48 and the Helicobacter hepaticus antigen P25Hh4. The present invention further provides methods and kits for detection and diagnosis of Helicobacter infections using the polynucleotides and polypeptides described herein.
Claims31
An isolated polynucleotide that hybridizes under stringent conditions to a sequence selected from the group consisting of:
- SEQ ID NO:1, nucleotides 25-1122 of SEQ ID NO:1, nucleotides 856-1962 of SEQ ID NO:1, nucleotides 1645-2799 of SEQ ID NO:
- 1, nucleotides 2560-3675 of SEQ ID NO:
- 1, nucleotides 3409-4632 of SEQ ID NO:
- 1, SEQ ID NO:12, SEQ ID NO:15, or a complement thereof.
An isolated polynucleotide that hybridizes under stringent conditions to SEQ ID NO:
- 1 or a complement thereof.
An isolated polynucleotide that hybridizes under stringent conditions to nucleotides 25-1122 of SEQ ID NO:
- 1 or a complement thereof.
An isolated polynucleotide that hybridizes under stringent conditions to nucleotides 856-1962 of SEQ ID NO:1 or a complement thereof.
An isolated polynucleotide that hybridizes under stringent conditions to nucleotides 1645-2799 of SEQ ID NO:1 or a complement thereof.
An isolated polynucleotide that hybridizes under stringent conditions to nucleotides 2560-3675 of SEQ ID NO:
- 1 or a complement thereof.
An isolated polynucleotide that hybridizes under stringent conditions to nucleotides 3409-4632 of SEQ ID NO:
- 1 or a complement thereof.
An isolated polynucleotide that hybridizes under stringent conditions to SEQ ID NO:12 or a complement thereof.
An isolated polynucleotide that hybridizes under stringent conditions to SEQ ID NO:
- 15 or a complement thereof.
A method for detecting Helicobacter infection in a rodent comprising the steps of:
- (a) contacting a polypeptide encoded by SEQ ID NO:
- 1, SEQ ID NO:
- 12, SEQ ID NO:
- 15 or an immunogenic fragment of said polypeptide with a biological sample, thereby forming a complex between the polypeptide and an antibody in the sample;
- and
- (b) detecting the presence of the complex, thereby detecting the presence of Helicobacter infection.
A kit for detecting Helicobacter infection comprising a polypeptide encoded by SEQ ID NO:1, SEQ ID NO:12, SEQ ID NO:15, or an immunogenic fragment of said polypeptide.
A method for detecting Helicobacter infection, said method comprising the steps of:
- (a) amplifying a target nucleic acid sequence with a first primer and a second primer specific for Helicobacter nucleotide sequence;
- and
- (b) detecting said amplified product.
Dependencies are inferred from the claim text.