Preparation and standardization of immunomodulatory peptide-linked glucans with verifiable oral absorbability from coriolus versicolor
AssigneeThe Chinese University of Hong Kong
InventorsAlbert H.L. Chow, Kevin K.W. Chu
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Abstract
This invention provides compositions and methods for stimulating the immune system. Such methods include administering an extract, purified peptide-linked glucan or active component thereof from Coriolus versicolor. The methods are particularly useful for prophylactic and therapeutic treatment of secondary immunodeficiency, wherein the immunodeficiency is the result of an infection, a malignant neoplastic disease, an autoimmune disease, a protein losing state, an immunosuppressive treatment, surgery or anesthesia.
Claims58
A purified extract of Coriolus versicolor comprising at least one peptide-inked glucan comprising glucose molecules linked by a (1→3) linkage, having a molecular weight of 0.3 kDa to 5 kDa as determined by size exclusion chromatography;
- and having immune stimulating activity.
An isolated peptide-linked glucan of Coriolus versicolor comprising a plurality of glucose molecules linked by a (1→3) linkage;
- a molecular weight of 0.7 kDa to 3.0 kDa as determined by size exclusion chromatography;
- and, the isolated peptide-linked glucan and an active component thereof have immune stimulating activity.
A pharmaceutical composition comprising the extract or isolated peptide-linked glucan of any one of the preceding claims.
A method of purifying a peptide-linked glucan from
- treating Coriolus versicolor with alkali, and separating a supernatant;
- subjecting the supernatant to cationic exchange;
- subjecting eluate from the cationic exchange to anionic exchange;
- subjecting eluate from the anionic exchange to a size fractionation technique, and collecting a fraction comprising peptide-linked glucan having a molecular weight of 0.7 to 5 kDa.
Dependencies are inferred from the claim text.