Methods for protection against Coccidioides spp. infection using Coccidioides spp. urea amidohydrolase (Ure) protein
OwnerGarry T. Cole, Jieh-Juen Yu +2inventors
InventorsGarry T. Cole, Jieh-Juen Yu, Kun Li, Kalpathi Seshan
Life of this patent
Estimating the term…
Abstract
The present invention provides compositions and methods of use of urea amidohydrolase (Ure) antigens and polynucleotides encoding the Ure antigens for generating an immunological response in an individual and in therapeutic and diagnostic applications of infections due to pathogenic Coccidioides spp. fungi, such as C. immitis or C. posadasii.
Claims31
A method of eliciting an immune response in a mammal, comprising introducing into the mammal, in an amount sufficient to elicit an immune response, an isolated polypeptide selected from the group consisting of:
- a polypeptide encoded by the nucleotide sequence of SEQ ID NO:1, a polypeptide comprised of the amino acid sequence of SEQ ID NO:2, a polypeptide encoded by the nucleotide sequence of nucleotides 100 to 1749 of SEQ ID NO:3, a polypeptide comprised of the amino acid sequence of amino acids 34 to 582 of SEQ ID NO:4, and a polypeptide comprised of the amino acid sequence of amino acids 34 to 582 of SEQ ID NO:4 with conservative amino acid substitutions.
The method of claim 2, wherein said mammal is a human.
A method of vaccinating a mammal from coccidioidomycosis, comprising administering into the mammal, in an amount sufficient to elicit an immune response wherein said immune response is sufficient to suppress or attenuate growth of Coccidioides spp. fungus in a mammal infected with said fungus, an isolated polypeptide selected from the group consisting of:
- a polypeptide encoded by the nucleotide sequence of SEQ ID NO:1, a polypeptide comprised of the amino acid sequence of SEQ ID NO:2, a polypeptide encoded by the nucleotide sequence of nucleotides 100 to 1749 of SEQ ID NO:3, a polypeptide comprised of the amino acid sequence of amino acids 34 to 582 of SEQ ID NO:4, and a polypeptide comprised of the amino acid sequence of amino acids 34 to 582 of SEQ ID NO:4 with conservative amino acid substitutions.
An isolated nucleic acid selected from the group consisting of:
- a nucleic acid comprising the nucleotide sequence of SEQ ID NO:1, a nucleic acid encoding the polypeptide comprising the amino acid sequence of SEQ ID NO:2, a nucleic acid comprising the sequence of nucleotides 100 to 1749 of SEQ ID NO:3, a nucleic acid encoding the polypeptide comprised of amino acids 34 to 582 of SEQ ID NO:4, and a nucleic acid encoding the polypeptide comprised of amino acids 34 to 582 of SEQ ID NO:4 with conservative amino acid substitutions.
An isolated polypeptide selected from the group consisting of:
- a polypeptide encoded by the nucleotide sequence of SEQ ID NO:1, a polypeptide comprised of the amino acid sequence of SEQ ID NO:2, a polypeptide encoded by the nucleotide sequence of nucleotides 100 to 1749 of SEQ ID NO:3, a polypeptide comprised of the amino acid sequence of amino acids 34 to 582 of SEQ ID NO:4, and a polypeptide comprised of the amino acid sequence of amino acids 34 to 582 of SEQ ID NO:2 with conservative amino acid substitutions.
A method of generating antibodies specific for Ure, comprising introducing into a mammal the isolated polypeptide comprised of the amino acid sequence of amino acids 34 to 582 of SEQ ID NO:4, in an amount sufficient to elicit an antibody response.
Dependencies are inferred from the claim text.